30,738 results

1. pNIC-CTHF

   Plasmid

   #26105

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Tags

   TEV - His6 - Flag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Stanley Qi, Carol Gross, Jason Peters

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   veg

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GSK3 beta pGEX

   Plasmid

   #15898

   Depositor

   Jim Woodgett

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 1, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMRE-Tn7-160

   Plasmid

   #118572

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1 p37

   Plasmid

   #169015

   Purpose

   Expresses GST-p37 (human, codon optimized for expression in bacteria)

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   p37 (UBXN2B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   sequence was codon optimized for expression in E.…

   Promoter

   tac

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExp-Lipo

   Plasmid

   #129237

   Purpose

   Protein expression in E.coli

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   His and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

   Plasmid

   #109995

   Purpose

   Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTHSSe_43

    Plasmid

    #109241

    Purpose

    pSC101 var1 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG/III-hs-MBP-CB2-HF

    Plasmid

    #121682

    Purpose

    Bacterial expression of cannabinoid receptor CB2 fusion protein

    Depositor

    Richard Henderson, Reinhard Grisshammer

    Article

    Calandra et al Biotechnology Letters

    Insert

    peripheral cannabinoid receptor (CNR2 Human)
    

    Tags

    His6 Flag tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28c S2

    Plasmid

    #171920

    Purpose

    Express RNA-based (p)ppGpp sensor

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Insert

    RNA-based (p)ppGpp sensor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTHSSe_44

    Plasmid

    #109242

    Purpose

    pSC101 var2 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Expression

    Bacterial

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP1B

    Plasmid

    #73241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1B (FKBP1B Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMRE-Tn5-157

    Plasmid

    #118539

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC101-pT7-T3RNAP

    Plasmid

    #140872

    Purpose

    T7 promoter driven synthesis of T3 RNA polymerase

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    T3 RNA polymerase
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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