32,963 results
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Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
ExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-BI-AR
Plasmid#171234PurposeMammalian expression of 2xHA-tagged human androgen receptor (hAR)DepositorInsert2xHA-tagged human androgen receptor (AR Human)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
ExpressionBacterialPromoterJ23101** and PBADAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorAvailable SinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAtetO_6xHis-Klentaq
Plasmid#157862PurposePlasmid for the aTc-inducible expression of the KlentaqDepositorInsertKlentaq
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOE7706.1
Plasmid#135075PurposeA shuttle vector for heterologous HA production with Corynebacterium glutamicumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
ExpressionBacterial and YeastMutationE366G (See depositor comment below)Available SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-loop
Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG87 FAST
Plasmid#130719PurposeExpresses His-tagged FAST (also called YFAST) in bacteriaDepositorInsertFAST
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyExpressionBacterialPromoterPem7 and PmAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyExpressionBacterialPromoterPem7Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
Plasmid#119945PurposeExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)
Plasmid#114073PurposeT7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tagDepositorInserthuman codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialMutationE174R, S542R, K548R and N282AAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-135
Plasmid#118553PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH532_MBP-Cas14b1 expression
Plasmid#112505PurposeExpresses 10xHis-MBP fused to Cas14b1 under t7 promoterDepositorInsertCas14b1
UseCRISPRTagsMBPExpressionBacterialPromoterT7Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
ExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAA3.stelN
Plasmid#112011PurposeExpresses TelN enzymeDepositorInsertTelN
Tagsstrep and hisExpressionBacterialPromoterT7Available SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCre-SacB-zeo
Plasmid#107706PurposeExpresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.DepositorInsertble (Zeo resistance)
ExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only