5,013 results

Showing: 271 - 300 of 5013 results

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pAC-14-LEXA-QF

Plasmid

#46104

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA-QF

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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13XLexAop2 IVS ChrimsonR mCherry_tr

Plasmid

#111548

Purpose

Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4

Plasmid

#187116

Purpose

insect cell expression of DDIT4

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Promoter

dHsp70

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-GFP

Plasmid

#175363

Purpose

ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Insert

sgGFP

Expression

Insect

Mutation

F64L, S65C, and I167T

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

Plasmid

#118412

Purpose

Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

Depositor

Nicole Paulk

Article

Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

Insert

Firefly luciferase

Use

AAV, Luciferase, and Synthetic Biology

Expression

Insect and Mammalian

Promoter

EF1a

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Human, Synthetic)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

Plasmid

#231721

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

POLR2A (POLR2A Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

RPB1:1586-1971

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

Plasmid

#185449

Purpose

Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF2 (TERF2 Human)
RAP1 (TERF2IP Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_FluA/H7N9

Plasmid

#229860

Purpose

pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

Depositor

Stephen Cusack

Article

Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

Inserts

PA_H7N9-FluA
PB1_H7N9-FluA
PB2_H7N9-FluA

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGBdest-GST-PI3Kc1

Plasmid

#187992

Purpose

Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

Depositor

Sascha Martens

Inserts

ATG14 (ATG14 Human)
VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)
BECN1 (BECN1 Human)

Tags

GST

Expression

Bacterial and Insect

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mMD2-proA

Plasmid

#187878

Purpose

baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

MD2 (Ly96 Mouse)

Expression

Insect

Mutation

includes residues 19–160 of mouse MD-2

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_774

Plasmid

#176673

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

Plasmid

#133560

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pVL1392 TMEM97

Plasmid

#157748

Purpose

Baculovirus transfer vectors to create a virus expressing human TMEM97

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Tags

flag

Expression

Insect

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-HLTF

Plasmid

#193055

Purpose

Baculovirus expression of human HLTF for recombinant protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

HLTF (HLTF Human)

Tags

3xFlag

Expression

Insect

Promoter

polyhedron

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159558

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCD8-GFP in pUAST

Plasmid

#17746

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

mouse CD8 (Cd8a Mouse)

Tags

GFP

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-sleeping beauty

Plasmid

#65487

Purpose

HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

Depositor

Guillaume Filion

Insert

sleeping beauty transposase

Expression

Insect

Promoter

HSP

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
R57C10-Flp2

Plasmid

#63172

Purpose

R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

yeast wild type containing a Glycine at amino acid 5 of the protein sequence

Expression

Insect

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHoY110_pAC8_hsDCAF5_FL

Plasmid

#249183

Purpose

Express DCAF5(full length) in Insect cells

Depositor

Eric Fischer

Article

Radko-Juettner et al Nature. 2024 Apr;628(8007):442-449. doi:

Insert

DCAF5 full length (DCAF5 Human)

Tags

Strep_BirA_short-linker-TEV

Expression

Insect

Mutation

N/A

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-GST-TBK1

Plasmid

#221331

Purpose

TBK1 protein overexpression in Sf9 insect cells

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TBK1 (Tbk1 Mouse)

Tags

6xHis-TEV-2xGST-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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