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Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINJ
Plasmid#26045DepositorInsertHisGST3C-lacZ
UseTagsMAHHHHHH_GST_LEVLFQGTExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFB-GST-IRP2
Plasmid#226621PurposeExpresses human IRP2 in insect cellsDepositorInsertIRP2 (IREB2 Human)
UseTagsGSTExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.RBBP4
Plasmid#125164PurposeExpresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertHistone-binding protein RBBP4 (RBBP4 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::Halo7
Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC5.1-dCry-V5His
Plasmid#136237PurposeProtein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionInsectMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorInsertmouse CD8 (Cd8a Mouse)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFL His-p97
Plasmid#113510PurposeExpression of His-p97 in insect cells (Baculovirus system)DepositorInsertp97 (VCP Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
TH1204-pOCC120-FUS_opt(NheI)
Plasmid#221892PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTagsMBP-PreScission and mGFP-PreScission-6xHisExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Fly, Human, Aequorea victoria)
UseTagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …PromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 PHR
Plasmid#160552PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 HR
UseTagsHexa hisExpressionInsectMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pESF140_HisSpy_DDB1∆B
Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…PromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQF2wWB
Plasmid#61313PurposeEnhancer trap version of QF2w, analogous to pGAWBDepositorInsertQF2w
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_FluA/H7N9
Plasmid#229860PurposepFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)DepositorInsertsPA_H7N9-FluA
PB1_H7N9-FluA
PB2_H7N9-FluA
UseTags2x-Streptag and 8xHis-tagExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLBac-FluB-trimer
Plasmid#229859PurposeInfluenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)
UseTags2x-Streptag and 8xHis-tagExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10
Plasmid#218866PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-DEST
Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneUseTags3x-FlagExpressionInsectMutationPromoterpolyhedronAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)
Plasmid#30123DepositorTypeEmpty backboneUseTagsHis6-MBP-N10-TEVExpressionInsectMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 TEV LIC cloning vector (5B)
Plasmid#30122DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUASattB-ERK-SPARK
Plasmid#106923PurposeExpress ERK-SPARK, an activity reporter of ERK in DrosophilaDepositorInsertERK-SPARK
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-attB
Plasmid#104880PurposeFor insertion of 5xQUAS-geneX transgenes using PhiC31 integrase systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
NHR1+2-Flag pBacPAK8-3
Plasmid#17324DepositorInsertNeuralized-like (Neurl1a Mouse)
UseTagsFLAGExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only