4,958 results
-
Plasmid#177329PurposeCo-expression of the human active Cdk1/cyclin B1/Cks1 kinase complexDepositorInsertsTags2 x Strep-tag and 8 x HisExpressionInsectPromoterp10 and polhAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]
Plasmid#240410PurposeFor Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)DepositorInsertsExpressionInsectPromoter3xP3 and Fibroin-L proximal promoter (Plodia inte…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
Tags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL010097-Cas9
Plasmid#100707PurposeThe promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL010097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL010097 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFp10
Plasmid#170460PurposeEarlyBac insect cell expression systemDepositorInsertnone
ExpressionInsectPromoterdHsp70Available SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
Tagshuman H2BExpressionInsectPromoter10xUASAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionInsectPromoterPolyhedronAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC-sleeping beauty
Plasmid#65487PurposeHSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.DepositorInsertsleeping beauty transposase
ExpressionInsectPromoterHSPAvailable SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
xenCGN-Myc-His
Plasmid#236505PurposeFor expression of Xenopus CGN in Insect CellsDepositorAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBFv-U6.2
Plasmid#138400PurposegRNA expression vector for DrosophilaDepositorTypeEmpty backboneExpressionInsectPromoterU6-2Available SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVV210
Plasmid#87628PurposeDrives Bxb1 expression in the D. melanogaster germ line.DepositorInsertBxb1
ExpressionInsectPromotervasaAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECIA14
Plasmid#47051PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tagsDepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsAlkaline Phosphatase (human, placental), Flag, HR…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC150-20XUAS-IVS-Flp1::PEST
Plasmid#32132DepositorInsertFlippase1::PEST
TagsPESTExpressionInsectMutationAspartic Acid at Amino Acid 5Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT-IMD-V5-His
Plasmid#63748PurposeExpresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertimmune deficiency (imd Fly)
TagsV5-6XHisExpressionInsectMutation*see comment belowPromoterpMTAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 WT
Plasmid#110127PurposeExpresses Flag-tagged human URH49 (DDX39A)DepositorAvailable SinceApril 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab beta Derp1-117
Plasmid#113639PurposeInsect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptideDepositorInsertI-Ab beta Derp1-117 (H2-Ab1 Mouse)
Tags6x His tag, fused to Der p 1 (117-127) peptide vi…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDK521
Plasmid#134204PurposeBaculovirus expresion of Astrin, LC8, and SKAPDepositorInsertAstrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
ExpressionInsectAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-3xHalo7::CAAX
Plasmid#87646PurposeFor creating drosophila transgenics expressing UAS-3xHalo7::CAAXDepositorInsert3xHalo7::CAAX
ExpressionInsectAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2::VP16Uw
Plasmid#26228DepositorInsertGAL4::VP16 (GAL4 Budding Yeast)
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINM
Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
TagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only