4,931 results

1. pAc5.1B-EGFP-DmCaf40-A253FY208A_X

   Plasmid

   #147973

   Purpose

   Insect Expression of DmCaf40-A253FY208A

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   DmCaf40-A253FY208A (Rcd-1 Fly)
   

   Tags

   EGFP (A179V)

   Expression

   Insect

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

   Plasmid

   #148160

   Purpose

   Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
   

   Expression

   Insect

   Mutation

   alias: 4E-T; one non silent mutation L127S, annot…

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-HsTNRC6C_L

   Plasmid

   #146855

   Purpose

   Insect Expression of HsTNRC6C

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsTNRC6C (TNRC6C Human)
   

   Expression

   Insect

   Mutation

   one non silent mutation Q1183K compard to the seq…

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ppBAalbNixE1E3E4 PUb-YFP

   Plasmid

   #173666

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   YFP
   

   Tags

   SV40 NLS

   Expression

   Insect

   Mutation

   Nix isoforms with exons E1, E3, E4

   Promoter

   Aedes aegypti PUb and own

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-HsTNRC6A_L

   Plasmid

   #146843

   Purpose

   Insect Expression of HsTNRC6A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsTNRC6A (TNRC6A Human)
   

   Expression

   Insect

   Mutation

   A339T, K1578E compared to the sequence of isoform…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

   Plasmid

   #147580

   Purpose

   Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)
   

   Expression

   Insect

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1C-FlucV5His6-PolyA-HhR_AB

   Plasmid

   #148303

   Purpose

   Insect Expression of PolyA-HhR

   Depositor

   Elisa Izaurralde

   Insert

   PolyA-HhR
   

   Expression

   Insect

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q

   Plasmid

   #147340

   Purpose

   Insect Expression of DmNot4-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   DmNot4-dsRNAres (CG31716 Fly)
   

   Expression

   Insect

   Mutation

   7 silent mutations and 6 non silent mutations G20…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-Dm4E-T-L36AL39A_T

   Plasmid

   #147604

   Purpose

   Insect Expression of Dm4E-T-L36AL39A

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   Dm4E-T-L36AL39A (4E-T Fly)
   

   Expression

   Insect

   Mutation

   Two non silent mutations (H628R and N659S) compar…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

    Plasmid

    #147279

    Purpose

    Insect Expression of DmNot3-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q

    Plasmid

    #147274

    Purpose

    Insect Expression of DmeIF4Ga-Y80A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
    

    Expression

    Insect

    Mutation

    one non silent mutation K340R compared to the seq…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid

    #147262

    Purpose

    Insect Expression of DmNot3-F818A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F818A-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmGW182-10xmut_G

    Plasmid

    #146428

    Purpose

    Insect Expression of DmGW182-10xmut

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

    Insert

    DmGW182-10xmut (gw Fly)
    

    Expression

    Insect

    Mutation

    Multiple mutations compared to NM_166780.1, see a…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P

    Plasmid

    #147209

    Purpose

    Insect Expression of DmXRN1-P1339AP1342A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

    Plasmid

    #146702

    Purpose

    Insect Expression of DmNot1_1710-2505

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmNot1_1710-2505 (Not1 Fly)
    

    Expression

    Insect

    Mutation

    two non silent mutations (A1747T, G1999S) and one…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmCup-Y342AL347A_O

    Plasmid

    #147103

    Purpose

    Insect Expression of DmCup-Y342AL347A

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmCup-Y342AL347A (cup Fly)
    

    Expression

    Insect

    Mutation

    Y342A, L347A, I350L, P418S, K1045N

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP-DmHPat-dsRNAres_J

    Plasmid

    #146692

    Purpose

    Insect Expression of DmHPat-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat-dsRNAres (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    two non silent, A479V, G568D and one silent mutat…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

    Plasmid

    #147394

    Purpose

    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Human, Nematode)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O

    Plasmid

    #147065

    Purpose

    Insect Expression of DmXRN1-D207A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmXRN1-D207A-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N

    Plasmid

    #146969

    Purpose

    Insect Expression of DmXRN1-del1323-1355-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmXRN1-del1323-1355-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-lambdaN-HA-DmGW182-delCterm_E

    Plasmid

    #146252

    Purpose

    Insect Expression of DmGW182-delC-term

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

    Insert

    DmGW182-delC-term (gw Fly)
    

    Expression

    Insect

    Mutation

    V758A, Q817R, and N853S

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E

    Plasmid

    #146241

    Purpose

    Insect Expression of DmSMG6-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

    Insert

    DmSMG6-siRNAres (Smg6 Fly)
    

    Expression

    Insect

    Mutation

    T151A, deleted A947

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAct-GST-H3(K27Q)

    Plasmid

    #182843

    Purpose

    a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    GST-histone H3 fusion
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAct-GST-H3

    Plasmid

    #182842

    Purpose

    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    GST-histone H3 fusion
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMT-FLAG-CBPΔ(N+HAT)

    Plasmid

    #182841

    Purpose

    An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP (w Fly)
    

    Tags

    FLAG tag

    Expression

    Insect

    Mutation

    dletion of residues 1566-2142 from CBP1476-3222

    Promoter

    MT

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Tags

    FLAG tag

    Expression

    Insect

    Promoter

    MT

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCasper-pNos-MCP-mCherry

    Plasmid

    #180275

    Purpose

    to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    MCP-mCherry
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    nanos

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p{3P3-mTurquoise::FRT,attB}

    Plasmid

    #182180

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    mTurquoise
    

    Expression

    Insect

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p{Mhc-F4-678-EGFP::GW,attB}

    Plasmid

    #182170

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Expression

    Insect

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p{3P3-mTurquoise::GW,attB}

    Plasmid

    #182169

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    mTurquoise
    

    Expression

    Insect

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 3xTRE DREaMR 3p3DsR

    Plasmid

    #182206

    Purpose

    3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    rTetR-3XP3-DsRed
    

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 1xTRE DREaMR 3p3DsR

    Plasmid

    #182204

    Purpose

    1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    rTetR-3XP3-DsRed
    

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

    Plasmid

    #146359

    Purpose

    Insect Expression of DmDCP2-PB

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2-PB (DCP2 Fly)
    

    Expression

    Insect

    Mutation

    Contains an in-frame insertion that adds a Q afte…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-HA-HsAGO2_E

    Plasmid

    #146224

    Purpose

    Insect Expression of HsAGO2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2 (AGO2 Human)
    

    Expression

    Insect

    Mutation

    Contains a K660E mutation compared to the sequenc…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

    Plasmid

    #146286

    Purpose

    Insect Expression of HsAGO2-F2V2

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    HsAGO2-F2V2 (AGO2 Human)
    

    Expression

    Insect

    Mutation

    Contains F470V, F505V, K660E mutations compared t…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-EGFP-DmHelz-E958Q_AH

    Plasmid

    #148884

    Purpose

    Insect Expression of DmHelz-E958Q

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz-E958Q (CG9425 Fly)
    

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pExpreS2-1-CR-PA

    Plasmid

    #175445

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    BiP Secretion Peptide and Thrombin-Protein A

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BicC.Ca9.R

    Plasmid

    #168295

    Purpose

    Expresses Cas9 in Drosophila late germline cells

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    BicC.Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 1137B=Hsp70Bb-Cas9

    Plasmid

    #153284

    Purpose

    The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 1046E=dgRNAs[traB,bTub]

    Plasmid

    #149425

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    U6.3-gRNAs[TraB, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6.3

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge