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Plasmid#157748PurposeBaculovirus transfer vectors to create a virus expressing human TMEM97DepositorInsertTMEM97 (TMEM97 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB-GST-3C-CDK1
Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
UseTagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-Cyclin-B
Plasmid#177012PurposeGenerate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEVDepositorInsertCyclin B1 (CCNB1 Human)
UseTagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINVL
Plasmid#26040DepositorInsertu-LacZ
UseTagsu-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACU2
Plasmid#31223DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINVH
Plasmid#26041DepositorInsertu-LacZ-KHHHHHH
UseTagsKHHHHHH and u-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-GFP
Plasmid#130277PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.DepositorInsert3xP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
UseTagsExpressionInsectMutationPromoterDrosophila metallothionein promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pUAST_mLExA-VP16-NFATc1 (C-del)
Plasmid#35023DepositorInsertNFATc1 (NFATC1 Human)
UseTagsVP16 and mLexAExpressionInsectMutationdeletion of amino acids 590-716PromoterUASAvailable sinceMarch 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-RFP
Plasmid#130276PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.DepositorInsert3xP3-RFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
Plasmid#55220DepositorTypeEmpty backboneUseTagsHis6 and MBP, Asn10ExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
Plasmid#185447PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-mMD2-proA
Plasmid#187878Purposebaculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tagDepositorInsertMD2 (Ly96 Mouse)
UseTagsExpressionInsectMutationincludes residues 19–160 of mouse MD-2PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJFRC165-20XUAS-IVS-R::PEST
Plasmid#32142DepositorInsertR::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF57/SMARCE1-His
Plasmid#177861PurposeTransfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His proteinDepositorInsertSMARCE1 (SMARCE1 Human)
UseTagsHis tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only