4,908 results

1. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac HT JS-Munc18c

   Plasmid

   #135555

   Purpose

   Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18c
   

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

   Plasmid

   #111903

   Purpose

   Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

   Depositor

   Andrew Carter

   Inserts

   His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
   DYNC1I2 (DYNC1I2 Human)
   DYNC1LI2 (DYNC1LI2 Human)
   DYNLT1 (DYNLT1 Human)
   DYNLRB1 (DYNLRB1 Human)
   DYNLL1 (DYNLL1 Human)
   

   Use

   Tags

   8xHis, SNAPf-tag, TEV site, and ZZ-tag

   Expression

   Insect

   Mutation

   Optimised for expression in Sf9 cells.

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc-C Med12His

   Plasmid

   #49240

   Purpose

   expresses human Med12 with His tag in insect cells

   Depositor

   Dylan Taatjes

   Article

   Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

   Insert

   Med12 (MED12 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. 57F03_LexA-GAD

   Plasmid

   #83256

   Purpose

   Expresses LexA-GAD under the control of 57F03 enhancer

   Depositor

   Leslie Vosshall

   Article

   Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

   Insert

   57F03 enhancer
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBPGUw-HACK-G4>QF2

   Plasmid

   #80277

   Purpose

   GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFD5-NS

   Plasmid

   #149546

   Purpose

   Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHsp83-NLS-HA-2xPCP-2xGFP

    Plasmid

    #71243

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    2xPCP-2xGFP
    

    Use

    P element-mediated germline transformation

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Hsp83

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPaint-T2A-Gal4-3xP3-RFP

    Plasmid

    #127556

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFBOT563

    Plasmid

    #40864

    Depositor

    Harold Gainer

    Article

    Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

    Insert

    Oxytocin promoter
    

    Use

    AAV

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    mouse oxytocin promoter

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBP-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199201

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psiCHECK2-let-7 8x

    Plasmid

    #20931

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

    Insert

    8x let-7 target sites
    

    Use

    Luciferase

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAct-dCBEevoCDA1

    Plasmid

    #195515

    Purpose

    Ubiquitous expression of dCBEevoCDA1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoCDA1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHY002_Strep_Avi_hsDCAF15

    Plasmid

    #174479

    Purpose

    Expresses DCAF15 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DCAF 15 (DCAF15 Human)
    

    Use

    Tags

    StrepII-Avi-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

    Plasmid

    #32149

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    FRT> STOP STOP FRT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOET5 rota-VLP

    Plasmid

    #218147

    Purpose

    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).

    Depositor

    Minna Hankaniemi

    Insert

    Rotavirus VP6
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge