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4,958 results

Showing: 951 - 1000 of 4958 results

  17. SPHK1

    Plasmid
    #73260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPHK1 (SPHK1 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73260-plasmid-map-sequence-id-137346

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  25. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  29. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Available Since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83342-plasmid-map-sequence-id-158671

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  32. pMT-TRAF6-V5-His

    Plasmid
    #63752
    Purpose
    Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TRAF6 (Traf6 Fly)
    Tags
    V5-His
    Expression
    Insect
    Promoter
    pMT
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63752-plasmid-map-sequence-id-106437

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  37. pMT-TAK1-V5-His

    Plasmid
    #63753
    Purpose
    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TGF-β activated kinase 1 (Tak1 Fly)
    Tags
    V5-His
    Expression
    Insect
    Promoter
    pMT
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63753-plasmid-map-sequence-id-106438

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  39. pOCC292

    Plasmid
    #118881
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    Tags
    mRuby3, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118881-plasmid-map-sequence-id-232308

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  40. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(dark)-6aa-DAD
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Available Since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    53096-plasmid-map-sequence-id-119521

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  43. pAc5.1B-EGFP-DmAtx2_AE

    Plasmid
    #148548
    Purpose
    Insect Expression of DmAtx2
    Depositor
    Elisa Izaurralde
    Insert
    DmAtx2 (Atx2 Fly)
    Expression
    Insect
    Mutation
    Three silent mutations and two deletions compared…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148548-plasmid-map-sequence-id-474981

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  45. QPRTA-c002

    Plasmid
    #205115
    Purpose
    QPRT protein expression
    Depositor
    Paul Brennan
    Insert
    QPRT (QPRT Human)
    Tags
    TEV-6His
    Expression
    Insect
    Mutation
    WT
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205115-plasmid-map-sequence-id-410409

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Showing: 951 - 1000 of 4958 results