5,017 results
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Plasmid#83343Purposefor Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only
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PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-QSco
Plasmid#46106DepositorInsertQSco
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-SAS6
Plasmid#80273Purposebaculovirus creation plasmid for GST-SAS6DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-GAL4QF
Plasmid#61309PurposeExpresses chimeric transactivator GAL4_DBD:QF2w_AD in DrosophilaDepositorInsertGAL4_DBD::QF2w_AD
ExpressionInsectMutationChanged EK on C-terminus of QF2 to KKKKAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP
Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRExpressionInsectAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-SBP_J
Plasmid#146621PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-V5-SBP_J
Plasmid#146633PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G1
Plasmid#40174DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-RLuc-A90-HhR_O
Plasmid#147073PurposeInsect Expression of A90-HhRDepositorInsertA90-HhR
ExpressionInsectMutationA127 instead of A90 (see comments below)Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneTags2xStrepIIExpressionInsectPromoterpolyhydrinAvailable SinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC6-15XUAS-IVS-mCD8::GFP
Plasmid#26219DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Agam_695
Plasmid#176668PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMO
Plasmid#85696Purposeexpression of GFP-SUMO1 in Drosophila cellsDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoVG-lexA
Plasmid#138405PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQG)-LexAp65
ExpressionInsectAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT EGFP SV40 pA
Plasmid#69911PurposeExpresses EGFP in DrosophilaDepositorInsertEGFP
ExpressionInsectPromoterMetallothionein Promoter (pMT)Available SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT Stat92E-GFP
Plasmid#126662PurposeInducible expression of Stat92E-GFP in S2 cellsDepositorInsertStat29E
ExpressionInsectAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2olig-VHH(GFP)
Plasmid#126023Purposeexpress RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHRolig-VHH(GFP)
TagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTubulin NLS-mCherry-LEXY
Plasmid#182597PurposeDrosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertmCherry
TagsLEXY and NLSExpressionInsectAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
KCNK2
Plasmid#73257PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertKCNK2 (KCNK2 Human)
TagsTEV-His10-FLAGExpressionInsectMutationuserdefined:Full_Length 1411PromoterPolyhedrinAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab18
Plasmid#31740DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.HA.p52
Plasmid#39219DepositorAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDW464
Plasmid#8845DepositorTypeEmpty backboneTagsbiotin acceptor peptideExpressionInsectAvailable SinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
Tags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACU
Plasmid#58373PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q62C-A140C
Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 …PromoterpolyhedringAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKF257
Plasmid#52533Purposeexpresses I-SceI nuclease in DrosophilaDepositorAvailable SinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII
Plasmid#217897PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tagDepositorTags2xSTIIExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7
Plasmid#217898PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)DepositorAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CORO1C:1-474
Plasmid#210920PurposeInsect expression of full-length human CORO1C. N-terminal 6X His tag with TEV cleavage.DepositorInsertCORO1C:M1-A474 (CORO1C Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQUAST-syb-spCFP1-10
Plasmid#73699PurposeNeuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spCFP1–10 chimera
ExpressionInsectAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Profilin WT
Plasmid#53509DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJFRC2-INS
Plasmid#26215DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SpyTag-β2AR-SpyTag
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
TagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
rNrx1beta(47-299)
Plasmid#20173DepositorAvailable SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pE1c
Plasmid#17461Purposemodified pENTR vector for C-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-M13::GAL4DBDo
Plasmid#64720Purposegenerating transgenic TRIC fliesDepositorInsertM13::GAL4DBDo
ExpressionInsectAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-Flag Ring1A
Plasmid#1995DepositorAvailable SinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EZH2
Plasmid#125171PurposeExpresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-myr::4xCLIPf
Plasmid#87635PurposeFor creating drosophila transgenics expressing UAS-myr4x::CLIPfDepositorInsertmyr::4xCLIPf
ExpressionInsectAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only