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4,958 results

Showing: 1151 - 1200 of 4958 results

  2. pUASt-GBP-Trbo

    Plasmid
    #179996
    Purpose
    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.
    Depositor
    Graydon Gonsalvez
    Article
    Baker et al Development. 2021 Nov 15;148(22). pii: 2
    Insert
    GBP nanobody
    Tags
    Trbo
    Expression
    Insect
    Available Since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179996-plasmid-map-sequence-id-357334

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  5. pE1c

    Plasmid
    #17461
    Purpose
    modified pENTR vector for C-terminal STREP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    STREP
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17461-plasmid-map-sequence-id-8629

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  18. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pUASp YFP Ras64B DN

    Plasmid
    #52243
    Purpose
    Drosophila expression of YFP-Ras64B dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    S19N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pUASp YFP Ras64B CA

    Plasmid
    #52244
    Purpose
    Drosophila expression of YFP-Ras64B constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q63L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pUASp YFP Ras64B WT

    Plasmid
    #52245
    Purpose
    Drosophila expression of YFP-Ras64B
    Depositor
    Matthew Scott
    Insert
    Ras64B (Ras64B Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Promoter
    GAL4
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  35. pBac[6xP3-Tc-hsp-ECFP-SV40]

    Plasmid
    #86447
    Purpose
    pBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)
    Depositor
    Gregor Bucher
    Insert
    6xP3-Tc-hsp-ECFP-SV40
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86447-plasmid-map-sequence-id-170877

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  37. DPYSL4 (DPYSL4A-c003)

    Plasmid
    #110263
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL4 (DPYSL4 Human)
    Tags
    His6-ZBasic-TEV
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110263-plasmid-map-sequence-id-212341

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  38. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. AAEL003877-Cas9

    Plasmid
    #100581
    Purpose
    The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL003877-hspcas9-GFP
    Opie-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Promoter
    AAEL003877 and Opie2
    Available Since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100581-plasmid-map-sequence-id-194325

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  41. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

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  43. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  48. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS3 (HAUS3 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS2 (HAUS2 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS6 (HAUS6 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS7 (HAUS7 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS4 (HAUS4 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS5 (HAUS5 Human, codon-optimized form for expression in Trichoplusia ni cells)
    HAUS8 (HAUS8 Human, codon-optimized form for expression in Trichoplusia ni cells)
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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Showing: 1151 - 1200 of 4958 results