5,038 results
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pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSAP
Plasmid#62293Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance markerDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKInt-hyg
Plasmid#18953DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
ExpressionInsectAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
Plasmid#81060Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_173
Plasmid#39830DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 mCit mCer
Plasmid#83563PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPRKCA (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_105_Venus_157
Plasmid#39819DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-EGFP
Plasmid#102782PurposeBaculovirus rescue vector, VSVGED pseudotype, eGFPDepositorInsertEGFP
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC8_GWs-Lox (VE5589)
Plasmid#163769PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.DepositorInsertno insert
Tagsno tagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy63
Plasmid#122015PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-QF#7-hsp70
Plasmid#46135DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorExpressionInsectAvailable SinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMFB2
Plasmid#31278DepositorAvailable SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKSB-611U6-sgRNA3 AGAG AACA
Plasmid#173673PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac{Mhc-453-GFP,attB,w+}
Plasmid#182199PurposeEGFP driven by 453bp enhancer of Mhc geneDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E2 (20–629)
Plasmid#132627Purposeexpresses human LRP6 E1E2 (20–629) in Hi5 insect cellDepositorInsertLRP6 (20–629)
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
rNL1(46-638)
Plasmid#20172DepositorInsertNeuroligin 1 (Nlgn1 Rat)
TagsC-terminal 6X His tagExpressionInsectMutationno splice sitesAvailable SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXb3
Plasmid#73615PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXb3
TagsmCherryExpressionBacterial, Insect, and Mamm…Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_AH
Plasmid#148918PurposeInsect Expression of DmGIGYFDepositorInsertDmGIGYF (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_641-1574_AH
Plasmid#148920PurposeInsect Expression of DmGIGYF_641-1574DepositorInsertDmGIGYF_641-1574 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGIGYF_1-640_AH
Plasmid#148919PurposeInsect Expression of DmGIGYF_1-640DepositorInsertDmGIGYF_1-640 (Gyf Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC120
Plasmid#118892Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEVDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and mGFP, HIS6, c…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pVL-FLAG/His PALI1
Plasmid#114449PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human PALI1 coding sequenceDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-IRES-let-7 8x
Plasmid#20932DepositorInsert8x let-7 target sites
UseLuciferaseExpressionInsect and MammalianMutation168bp IRES from 5'UTR of reaper gene in Dros…Available SinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
U6b-T
Plasmid#117210PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6b promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_159_Venus_173
Plasmid#39825DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_195
Plasmid#39831DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMos-3xP3DsRed-attP
Plasmid#52904PurposeMos1 transformation vector containing MCS, loxP, attP, 2 x 5HEDepositorTypeEmpty backboneExpressionInsectAvailable SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRB34
Plasmid#72862Purposetemplate for N-terminal 3x FLAG tag, constitutive Blasticidin resistanceDepositorInsert3xFlag tag
ExpressionInsectAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
Tags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
TagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
OA-1045C
Plasmid#125005PurposeExpresses gRNAs targeting hid, eve, and winglessDepositorAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-QF#7m1
Plasmid#46126DepositorInsertQF#7m1
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4
Plasmid#138392PurposeRMCE vector for generating C-terminal T2A-GAL4 fusionDepositorInsertT2A-GAL4
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-mCherry
Plasmid#126667PurposeInducible expression of Draper-mCherry in S2 cellsDepositorInsertDraper mCherry
ExpressionInsectMutationC813Y- see depositor comments belowAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM394
Plasmid#134211PurposeBaculovirus expression of CENP-N and CENP-LDepositorInsertCENP-N-his-CENP-L
ExpressionInsectAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-Myc-dATG1
Plasmid#111801PurposeInsect Expression of dAtg1DepositorAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-GST-Nter-GWs-Lox (VE5586)
Plasmid#163766PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.DepositorInsertN-terminal GST tag
TagsGST TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
TagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
T6-pAC5.1 mEGFP-EcoRV
Plasmid#206489PurposeSchneider cell imagingDepositorTypeEmpty backboneTagsmEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
