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Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pP{ELAV-GeneSwitch}
Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertUseTagsExpressionInsectMutationPromoterelavAvailable sinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
UseTags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorInsertLOV2
UseTagsNTOM20ExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAcBac2.tR4-OMeYRS/GFP*
Plasmid#50831Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette
UseBaculoviralTagsHis, Myc-6xHis, and WPREExpressionInsectMutationY39TAG and able to charge various unnatural amino…PromoterCAG, CMV, and U6 and H1Available sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
H9 F42A pAcGP67a
Plasmid#41810DepositorInsertH9 Super-2 (IL2 Human)
UseTags8x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-PSMA
Plasmid#11524DepositorInsertprostate-specific membrane antigen (FOLH1 Human)
UseTagsHisExpressionInsectMutationThere is a short N-terminal truncation of this pr…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pC-sleeping beauty
Plasmid#65487PurposeHSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.DepositorInsertsleeping beauty transposase
UseTagsExpressionInsectMutationPromoterHSPAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFp10
Plasmid#170460PurposeEarlyBac insect cell expression systemDepositorInsertnone
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5_w
Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-Bip-GFP:V5:KDEL
Plasmid#69917PurposeAn endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.DepositorInsertGFP
UseTagsDrosophila BiP signal sequence, KDEL ER targeting…ExpressionInsectMutationPromoterpMTAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralTagsExpressionInsectMutationPromoterCMV and U6 and H1Available sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCDMp10
Plasmid#170461PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-635
Plasmid#159582PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPLp10
Plasmid#170462PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
UseTagsHistidineExpressionBacterial, Insect, and Mamm…MutationPromoterCMV ie enhancer/promoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB_GST-TEV-SRC (Y530F)
Plasmid#223743PurposeExpression of recombinant protein for purificationDepositorInsertSrc Kinase (SRC Human)
UseTagsGST-TEVExpressionInsectMutationY530F constitutively active mutantPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
UseTagssmGdP-10XOllasExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-blast
Plasmid#208033PurposeEnables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoCDA1
Plasmid#195515PurposeUbiquitous expression of dCBEevoCDA1 in DrosophilaDepositorInsertdCBE-evoCDA1
UseCRISPRTagsExpressionInsectMutationPromoteract5CAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB_GST-TEV-SRC(WT)
Plasmid#223742PurposeExpression of recombinant protein for purification, codon optimized for Insect cellsDepositorInsertSrc Kinase (SRC Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorInsertHook3-SNAPf (HOOK3 Human)
UseTags8His-ZZ-LTLT and SNAPfExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only