4,932 results

1. rNrx1beta(47-299)

   Plasmid

   #20173

   Depositor

   Axel Brunger

   Article

   Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

   Insert

   Neurexin 1 beta (Nrxn1 Rat)
   

   Tags

   6X His tag

   Expression

   Insect

   Mutation

   no splice site

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pBac[6xP3-Tc-hsp-ECFP-SV40]

   Plasmid

   #86447

   Purpose

   pBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)

   Depositor

   Gregor Bucher

   Insert

   6xP3-Tc-hsp-ECFP-SV40
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2Bac.Myc.p44

   Plasmid

   #39220

   Depositor

   Aziz Sancar

   Insert

   p44 (GTF2H2 )
   

   Tags

   Myc

   Expression

   Insect

   Promoter

   Pp10

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pKSB-611U6-sgRNA3 AGAG AACA

   Plasmid

   #173673

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

   Plasmid

   #169185

   Purpose

   Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   His6-3xFlag (nsp12 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pF3BGX-TangoL-lexA

   Plasmid

   #138404

   Purpose

   RMCE vector for generating C-terminal Tango fusion

   Depositor

   Shu Kondo

   Article

   Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

   Insert

   TEVcs(ENLYFQL)-LexAp65
   

   Expression

   Insect

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBac{Mhc-453-GFP,attB,w+}

   Plasmid

   #182199

   Purpose

   EGFP driven by 453bp enhancer of Mhc gene

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   EGFP
   

   Expression

   Insect

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAEL003877-Cas9

   Plasmid

   #100581

   Purpose

   The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL003877-hspcas9-GFP
   Opie-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Promoter

   AAEL003877 and Opie2

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASp YFP Rac1 DN

   Plasmid

   #52234

   Purpose

   Drosophila expression of YFP-Rac1 dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Rac1 (Rac1 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T17N; DN mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAFMW-AthHEN1

    Plasmid

    #104980

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Tags

    FLAG Myc

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid

    #157813

    Purpose

    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

    Depositor

    Zoltan Lipinszki

    Article

    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

    Insert

    GFP-binding protein
    

    Use

    For drosophila cells

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    metallothionein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAS-kkv::NG

    Plasmid

    #138953

    Purpose

    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv (NEWENTRY Fly)
    

    Use

    Uas transgene vector

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Available Since

    Nov. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaSpeR4 mito roGFP2-Grx1

    Plasmid

    #65000

    Purpose

    Expression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-EGFP-DmGIGYF_AH

    Plasmid

    #148918

    Purpose

    Insect Expression of DmGIGYF

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-EGFP-DmGIGYF_641-1574_AH

    Plasmid

    #148920

    Purpose

    Insect Expression of DmGIGYF_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_641-1574 (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-EGFP-DmGIGYF_1-640_AH

    Plasmid

    #148919

    Purpose

    Insect Expression of DmGIGYF_1-640

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_1-640 (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRB39

    Plasmid

    #72870

    Purpose

    template for N-terminal eGFP tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT Lifeact-TagBFP

    Plasmid

    #126664

    Purpose

    Inducible expression of Lifeact-TagBFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Lifeact-TagBFP fusion
    

    Expression

    Insect

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac GST-Plk4

    Plasmid

    #80264

    Purpose

    baculovirus creation plasmid for GST-Plk4

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac1 PIK3R1-Ni

    Plasmid

    #17744

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1-Ni (PIK3R1 Human)
    

    Expression

    Insect

    Mutation

    Partial cDNA

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. gp64-mVEGF-C-HisTag-pENTR

    Plasmid

    #64065

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Tags

    His-tag

    Expression

    Insect

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRB40

    Plasmid

    #72865

    Purpose

    template for N-terminal eGFP tag, constitutive Blasticidin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    eGFP
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOPINO_F2

    Plasmid

    #171926

    Purpose

    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_F2
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pBMPGAL4LWL

    Plasmid

    #26270

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)-Rab10-FLAG

    Plasmid

    #135559

    Purpose

    Express mouse Rab10 in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    T7 promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. U6-BbsI-crRNA

    Plasmid

    #51026

    Purpose

    Generates crRNA for use in combination with tracrRNA

    Depositor

    Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

    Insert

    U6-BbsI-crRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6-2

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOCC114

    Plasmid

    #118904

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with TEV protease, two glycines

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQUAS-DSCP-FLPo

    Plasmid

    #24358

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    FLPo
    

    Expression

    Insect

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LexAop2-myr::4xSNAPf

    Plasmid

    #87638

    Purpose

    For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Expression

    Insect

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

    Plasmid

    #133560

    Purpose

    Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ac5-STABLE1-puro

    Plasmid

    #208031

    Purpose

    Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIHEU_miniSOG2 T2A H3.3A-EGFP

    Plasmid

    #87411

    Purpose

    UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

    Insert

    mini singlet oxygen generator 2
    

    Expression

    Insect

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLib6.4-Aalb_744

    Plasmid

    #176663

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUAST mito roGFP2-Grx1

    Plasmid

    #64995

    Purpose

    for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

38. CapChR2_pUAST-attb

    Plasmid

    #188041

    Purpose

    Expresses CapChR2 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2
    

    Tags

    none

    Expression

    Insect

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    UAS

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCFD3-Act5c

    Plasmid

    #130278

    Purpose

    Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    pCFD3-Act5c (Act5C Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Insect

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge