4,958 results
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Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-dsRed::FRT-RC,attB}
Plasmid#182177Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-eGFP::FRT-RC,attB}
Plasmid#182175Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT-RC,attB}
Plasmid#182181Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-16
Plasmid#104966PurposePlasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAsDepositorInsertRibozyme-gRNA-ribozyme
UseCre/LoxExpressionInsectAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-6His-Nter-GWs-Lox (VE5587)
Plasmid#163768PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.DepositorInsertN-terminal 6His tag
Tags6 His TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-10His-Cter (VE5631)
Plasmid#161802PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.DepositorInsertC-terminal 10 His tag
Tags10 HisExpressionInsectPromoterp10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-10His-Cter (VE5742)
Plasmid#139776PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.DepositorInsertC-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-6His-Cter (VE5630)
Plasmid#139777PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.DepositorInsertC-terminal 6His tag
Tags6 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-c-Myc-Cter (VE5632)
Plasmid#139778PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.DepositorInsertC-terminal C-Myc tag
Tagsc-MycExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-Flag-Cter(VE5738)
Plasmid#161798PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.DepositorInsertC-terminal Flag tag
TagsFlagExpressionInsectPromoterp10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-pH-Flag-Cter (VE5739)
Plasmid#161799PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.DepositorInsertC-terminal Flag tag
TagsFlagExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)
Plasmid#161800PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.DepositorInsertN-terminal HA tag
TagsHAExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-10His-Nter-GWs-Lox (VE5588)
Plasmid#161806PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.DepositorInsertN-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh PH-mCh (VE5625)
Plasmid#139769PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.DepositorInsertmCherry fluorescent protein
ExpressionInsectPromoterPH or p10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:bmp4-pPH:vsvged
Plasmid#111157PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInserthBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianMutationwtPromoterCMVAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASp dsRed Rab5 CA
Plasmid#53467DepositorAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM744
Plasmid#134225PurposeBaculovirus expression of CENP-T and CENP-WDepositorInserthis-CENP T + CENP W
ExpressionInsectAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM499
Plasmid#134216PurposeBaculovirus expression of CENP-U CENP-Q CENP-OPDepositorInserthis-CENPU + CENPQ+ CENPOP
ExpressionInsectAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC159-1XUAS-DSCP-B3::PEST
Plasmid#32137DepositorInsertB3::PEST
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
Plasmid#159692PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationM188LAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CG10077-RA_B
Plasmid#145910PurposeInsect Expression of CG10077-RADepositorInsertCG10077-RA (CG10077 Fly)
ExpressionInsectMutationA23T, Q440R, S483L, I492T, inserted G810, H672Y, …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CG10077-RB_B
Plasmid#145911PurposeInsect Expression of CG10077-RBDepositorAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CJ53 (cg5166a/c utr)
Plasmid#17781DepositorInsertcg5166a/c utr fragment
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectAvailable SinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ54 (cg5166a/c utr mut)
Plasmid#17782DepositorInsertcg5166a/c utr fragment mut
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationmutant versionAvailable SinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Moe-pDONR221
Plasmid#124239PurposeGateway entry clone of Drosophila Moesin cDNA encoding the D isoform.DepositorInsertDrosophila Moesin (Moe Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_195
Plasmid#40002DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-GAL4DBD
Plasmid#26264DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
HS-H4-GFP
Plasmid#8699DepositorAvailable SinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
Or30a-Gal4
Plasmid#63027PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or30a promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACSV-Ptc
Plasmid#37675DepositorAvailable SinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
p13xLexAOP2-IVS-Syn21-Voltron-p10
Plasmid#119042PurposeLexAop2-driven expression of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Jag1ICD-HA pBacPAK8-3
Plasmid#17331DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3H
Plasmid#49050PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionInsectPromoterpolH (polyhedrin)Available SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFBXNH3
Plasmid#49049PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionInsectPromoterpolH (polyhedrin)Available SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-mCherry
Plasmid#102781PurposeBaculovirus rescue vector, VSVGED pseudotype, mCherryDepositorInsertmCherry
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSK24
Plasmid#72852Purposetemplate for C-terminal Twin-Strep tag, Puromycin-resistanceDepositorInserttwin Strep tag
ExpressionInsectAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pfast NPC1(NTD)
Plasmid#25773DepositorAvailable SinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb AaegOr4-E
Plasmid#59796PurposeUAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele EDepositorInsertAedes aegypti Or4 cDNA allele E
ExpressionInsectAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
bTub-GFP.766A
Plasmid#112695Purposeexpressed GFP in testes, under bTub promoterDepositorInsertbTub-GFP-SV40
ExpressionInsectPromoterbTubAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
Plasmid#71812PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716Available SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
TagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS337-USP44-FLAG
Plasmid#43905DepositorAvailable SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
RIPK2 (RIPK2A-c036)
Plasmid#110268PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2_G674A
Plasmid#134874PurposeExpression human MSH2 G674 mutant protein using baculovirusDepositorInserthuman MSH2_G674A (MSH2 Human)
ExpressionInsectMutationChanged Glycine to Alanine at aa#674Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-QF
Plasmid#26267DepositorAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
AT5G48740 O3_pECIA2
Plasmid#114963PurposeBait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.DepositorInsertAT5G48740 (AT5G48740 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only