4,932 results
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Hy_pMT Flag-Hel25E WT
Plasmid#110123PurposeExpresses Flag-tagged D. melanogaster Hel25EDepositorAvailable SinceOct. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-Pav-GFP
Plasmid#24286DepositorAvailable SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pattB-DSCP-QF-sv40
Plasmid#24369DepositorInsertQF
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
RBBP4
Plasmid#25350PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRBBP4-13 (RBBP4 Human)
UseBaculovirus expressionTagsHisExpressionInsectPromoterpolyhedrinAvailable SinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP
Plasmid#32143DepositorInsertRSRT> STOP STOP RSRT>
ExpressionInsectAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac flag Mel18
Plasmid#1968DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKSB-631 U6-sgRNA3/4 AACA GCTT
Plasmid#173675PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-Myc-dATG1
Plasmid#111801PurposeInsect Expression of dAtg1DepositorAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-GST-Nter-GWs-Lox (VE5586)
Plasmid#163766PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.DepositorInsertN-terminal GST tag
TagsGST TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 2x
Plasmid#20929DepositorInsert2x let-7 target sites
UseLuciferaseExpressionInsect and MammalianAvailable SinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pVL-FLAG/His PALI1
Plasmid#114449PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human PALI1 coding sequenceDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC158-3XUAS-IVS-B3::PEST
Plasmid#32138DepositorInsertB3::PEST
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-EGFP
Plasmid#102782PurposeBaculovirus rescue vector, VSVGED pseudotype, eGFPDepositorInsertEGFP
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC120
Plasmid#118892Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEVDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and mGFP, HIS6, c…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMFB2
Plasmid#31278DepositorAvailable SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
AT5G62710 X045_pECIA2
Plasmid#115130PurposeBait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.DepositorInsertAT5G62710 (AT5G62710 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4
Plasmid#138392PurposeRMCE vector for generating C-terminal T2A-GAL4 fusionDepositorInsertT2A-GAL4
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Cquin_596
Plasmid#176655PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKInt-hyg
Plasmid#18953DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
ExpressionInsectAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-QF#7-hsp70
Plasmid#46135DepositorInsertQF#7
ExpressionInsectAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSAP
Plasmid#62293Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance markerDepositorTypeEmpty backboneExpressionInsectAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEGFP-NLS
Plasmid#176681PurposeExpression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEGFP
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM394
Plasmid#134211PurposeBaculovirus expression of CENP-N and CENP-LDepositorInsertCENP-N-his-CENP-L
ExpressionInsectAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-VAV2 DH/PH/C1
Plasmid#81060Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB34
Plasmid#72862Purposetemplate for N-terminal 3x FLAG tag, constitutive Blasticidin resistanceDepositorInsert3xFlag tag
ExpressionInsectAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorExpressionInsectAvailable SinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSAY-vasaCas9sv40
Plasmid#173669PurposeCas9 expression vector for AnophelesDepositorInsertCas9 under control of vasa promoter and SV40 terminator
UseCRISPRExpressionInsectPromotervasaAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPromoterless-P2A
Plasmid#165790PurposeViral P2A part in promoter grammar, for building promoterless control vectorsDepositorInsertPromoterless-2A
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169184PurposeBaculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cellsDepositorInsertnsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
TagsHis6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only
