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Plasmid#41806DepositorInsertIL2 (IL2 Human)
UseTags6x HisExpressionInsectMutationPromoterPolyhedronAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E2 PUb-GFP
Plasmid#173667Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
EGFP
UseTagsExpressionInsectMutationNix isoforms with only exons 1, 2 separated by in…PromoterAedes aegypti Polyubiquitin and cloned Nix promot…Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM50
Plasmid#123656PurposeEncodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cellsDepositorInsertAedes aegypti UbL40 promoter
UseLuciferaseTagsExpressionInsectMutationPromoterUbL40 promoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectMutationPromoterbhspAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsUseTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC157-20XUAS-IVS-B3::PEST
Plasmid#32136DepositorInsertB3::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-LexGAD-3xP3-RFP
Plasmid#127558PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertLexGAD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR TPP1 MBP TEV TIN2
Plasmid#185446PurposeCoexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4DBD-3xP3-RFP
Plasmid#127564PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4DBD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQUASp-mCD8GFP
Plasmid#46163PurposeExpression of murine CD8GFP in insectsDepositorInsertmCD8GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
p(U6b-BsaI-gRNA)
Plasmid#65956PurposeTribolium U6b promoter with BsaI cloning site to insert guide RNA sequenceDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterU6Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6
Plasmid#198408PurposeExpresses FLAG-tagged human IntS6DepositorInsertDDX26 (INTS6 Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::7xHalo7
Plasmid#87650PurposeFor creating drosophila transgenics expressing UAS-Syt::7xHalo7DepositorInsertSyt::7xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKSB-609 U6-sgRNA1 ATCC GGAA
Plasmid#173671PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-DsRedSV40 GGGG AAGA
Plasmid#174532Purpose3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-DsRed BsaI cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-mTurqSV40 GGGG AAGA
Plasmid#174533Purpose3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2olig-VHH(GFP)
Plasmid#126023Purposeexpress RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHRolig-VHH(GFP)
UseTagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only