4,908 results

1. R979-X58-008: Hs.KRAS4b(1-188)

   Plasmid

   #159691

   Purpose

   Baculovirus protein expression of KRAS4b (1-188)

   Depositor

   Dominic Esposito

   Article

   Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

   Insert

   Hs.KRAS4b(1-188) (KRAS Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1_GI.1_N116C-G193C

   Plasmid

   #162580

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Asparagine 116 was mutated to Cysteine and Glycin…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ANO10 (TMEM16KA-c001)

   Plasmid

   #110267

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TMEM16K (ANO10 Human)
   

   Use

   Tags

   TEV-His10-Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1043

   Plasmid

   #164586

   Purpose

   clone in U6 and DR

   Depositor

   Omar Akbari

   Article

   Sun et al J Vis Exp. 2021 Feb 5;(168). doi: 10.379

   Insert

   U6 and DR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEBac1-GCP4

   Plasmid

   #178070

   Purpose

   Insect cell expression of GCP4

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   GCP4 (TUBGCP4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. vas-Cas9.874Z

   Plasmid

   #112687

   Purpose

   Express hSpCas9 under vasa promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   vasa

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTV-Gal4DBD2xHighMag1

   Plasmid

   #182023

   Purpose

   Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

   Depositor

   Jean-Paul Vincent

   Article

   di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

   Insert

   Gal4DBD2xHighMag1
   

   Use

   Donor plasmid

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJFRC157-20XUAS-IVS-B3::PEST

    Plasmid

    #32136

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B3::PEST
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. Vector 1174D

    Plasmid

    #200012

    Purpose

    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

    Depositor

    Omar Akbari

    Article

    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

    Insert

    Engineered dobulesex splicing module
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Engineered dobulesex splicing module

    Promoter

    Hr5IE1

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFBD-Akt1(E17K)

    Plasmid

    #86563

    Purpose

    Expresses human full-length Akt1(E17K) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    E17K mutation in the PH domain

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-mCherry-Patronin

    Plasmid

    #31753

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFBD-ESCRT 0

    Plasmid

    #21499

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
    

    Use

    Tags

    GST and His-MBP

    Expression

    Insect

    Mutation

    none

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Use

    Tags

    3x HA, V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQUASp-RedStinger (nlsRFP)

    Plasmid

    #46165

    Purpose

    Expression of RedStinger in insect

    Depositor

    Christopher Potter

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid

    #172915

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7f-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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