4,932 results

1. pUASp YFP Cdc42 CA

   Plasmid

   #52250

   Purpose

   Drosophila expression of YFP-Cdc42 constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Cdc42 (Cdc42 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pQUAST-nucLacZ

   Plasmid

   #24356

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   LacZ (lacZ )
   

   Tags

   8 aa nuclear localization signal

   Expression

   Insect

   Available Since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-His-zfCsf1a

   Plasmid

   #168103

   Purpose

   Express soluble His-tagged zebrafish Csf1a in insect cells

   Depositor

   Petr Bartunek

   Article

   Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

   Insert

   Csf1a (csf1a Zebrafish)
   

   Tags

   6x His

   Expression

   Insect

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRIV-Gal4DBD:2xnMagHigh1

   Plasmid

   #182031

   Purpose

   Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

   Depositor

   Jean-Paul Vincent

   Article

   di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

   Insert

   Gal4DBD2xHighMag1-p103'UTR
   

   Use

   Donor plasmid

   Expression

   Insect

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-His-Flag-Tipin

   Plasmid

   #22890

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Tipin (TIPIN Human)
   

   Tags

   Flag- and His

   Expression

   Insect

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

   Plasmid

   #124069

   Purpose

   CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)

   Depositor

   Gregor Bucher

   Insert

   eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
   

   Use

   CRISPR and Cre/Lox

   Expression

   Insect

   Promoter

   bhsp68

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ORCO-T2A-QF2

   Plasmid

   #162524

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
   

   Expression

   Insect

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx4-ADCY6-C2 (917-1165 aa)

   Plasmid

   #113904

   Purpose

   His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

   Depositor

   Anjaparavanda Naren

   Article

   Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

   Insert

   Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
   

   Tags

   His-S

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Please see Depositor Comments

   Promoter

   T7, CMV

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx4-ADCY6-C1(306-672 aa)

    Plasmid

    #113903

    Purpose

    His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
    

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7, CMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT-HGP-v3-Nb127D01-hIgG

    Plasmid

    #171564

    Purpose

    Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
    

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMT-HGP-v3-NbVHH05-hIgG

    Plasmid

    #171565

    Purpose

    Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
    

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB-LIC C

    Plasmid

    #62288

    Purpose

    Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus

    Tags

    8XHis tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pFastBacI/MDR3cysless

    Plasmid

    #74400

    Purpose

    expression of MDR3 in insect cells

    Depositor

    Robert Nakamoto

    Insert

    MDR3 (Abcb1a Mouse)
    

    Tags

    10His

    Expression

    Insect

    Mutation

    all Cys to Ala

    Promoter

    Polyhedrin

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pUASp-Egl-Trbo

    Plasmid

    #179993

    Purpose

    Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    Egl-Trbo
    

    Tags

    TurboID

    Expression

    Insect

    Promoter

    UAS-Gal4

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mCherry-LOV2 V416T

    Plasmid

    #81040

    Purpose

    mammalian expression of LOV V416T

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2 V416T
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416T

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINF Ta-sro1 WWE-PARP

    Plasmid

    #192550

    Purpose

    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    

    Tags

    His6, 3C protease cleavage site

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7 promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CJ52 (cg1849 utr mut)

    Plasmid

    #17780

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg1849 utr fragment mut
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    mutant version

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCaST-dVP16AD

    Plasmid

    #15306

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    dVP16AD-ZIP+
    

    Expression

    Insect

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-ZZ-TEV-BICDR1

    Plasmid

    #111859

    Purpose

    Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, TEV site, and ZZ

    Expression

    Insect

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVMG0146_Cq-U6-1_2xBbsI-gRNA

    Plasmid

    #169238

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    Cq-U6-1_2xBbsI-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039653

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVMG0149_Cq-U6-6_2xBbsI-gRNA

    Plasmid

    #169323

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0149_Cq-U6-6_2xBbsI-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CPIJ039596

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BacPAK Flag-NFRKB

    Plasmid

    #19791

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pRB33

    Plasmid

    #72867

    Purpose

    template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    3xFlag tag
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Jag2ICD-HA pBacPAK8-3

    Plasmid

    #17332

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged2 (Jag2 Rat)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pBacPak-HA-E(y)2

    Plasmid

    #52288

    Purpose

    Expresses Drosophila E(y)2

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    E(y)2 (e(y)2 Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKM610

    Plasmid

    #134218

    Purpose

    Baculovirus expression of CENP-R

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-R untagged
    

    Expression

    Insect

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSK25

    Plasmid

    #72853

    Purpose

    template for C-terminal double-FLAG tag, Puromycin-resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    double Flag
    

    Expression

    Insect

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBDPR

    Plasmid

    #61598

    Purpose

    Transformation vector pBDPR

    Depositor

    John Carlson

    Article

    Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

    Type

    Empty backbone

    Use

    Drosophila transformation vector

    Expression

    Insect

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJC-mhc2.4kb-TALE3-VP64-P10

    Plasmid

    #104611

    Purpose

    Expresses TALE3 under the control of a 2.4kb mhc enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    2.4kb mhc enhancer element (Mhc Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

    Plasmid

    #71810

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]
    

    Expression

    Insect

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 44 pLIB_12HIS

    Plasmid

    #160186

    Purpose

    N-term 3C cleavable 12xHis tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBiex1-SYNAC

    Plasmid

    #82710

    Purpose

    Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

    Insert

    SYNAC (ADCY2, ADCY5 Human)
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Bacterial and Insect

    Promoter

    T7

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDW445

    Plasmid

    #8843

    Depositor

    David Waugh

    Article

    Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

    Type

    Empty backbone

    Tags

    biotin acceptor peptide

    Expression

    Insect

    Available Since

    Nov. 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKAR2G_design1_mTFP_wt_Venus_157

    Plasmid

    #39814

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

36. ULK3 (ULK3A-c002)

    Plasmid

    #110272

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ULK3 (ULK3 Human)
    

    Tags

    His6-ZBasic-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSK15

    Plasmid

    #72855

    Purpose

    template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    attP site
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUWR-CadTS

    Plasmid

    #58325

    Purpose

    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    E-cadherin tension sensor
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pAc5.1B-lambdaN-HA-CeALG1_O

    Plasmid

    #147147

    Purpose

    Insect Expression of CeALG1

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

    Insert

    CeALG1
    

    Expression

    Insect

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFastBac-Giardia Dicer

    Plasmid

    #89146

    Purpose

    Expression of Giardia Dicer in Sf9 cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    Giardia Dicer
    

    Tags

    His6-teve

    Expression

    Insect

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge