5,017 results

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pJFRC19-13XLexAop2-IVS-myr::GFP

Plasmid

#26224

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

myr::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9L

Plasmid

#166788

Purpose

Insect cell expression vector for DPP9L (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

Plasmid

#200400

Purpose

Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

Depositor

Daniel Kronauer

Article

Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

Inserts

Discosoma red fluorescent protein
Q factor 2
GCaMP6s

Expression

Insect

Promoter

15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#5

Plasmid

#176337

Purpose

The synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#5

Expression

Insect

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-LOV2

Plasmid

#81009

Purpose

Expression of LOV2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

NTOM20

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-dU6:3gRNA

Plasmid

#49410

Purpose

expression of gRNA under control of the Drosophila U6:3 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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FoRC.attBGm

Plasmid

#84588

Purpose

Drosophila PhiC31 transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pFastBac1-pGC-A

Plasmid

#186626

Purpose

pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

Depositor

Debra Hansen

Article

Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

Insert

NPR1 (NPR1 Human)

Tags

hemagglutinin (HA) signal peptide + His10-tag + T…

Expression

Insect

Mutation

deleted amino acids 1-32

Promoter

polyhedring

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacHTa_HCK

Plasmid

#224245

Purpose

Expression of human HCK Kinase in insect cells (SF9 cells)

Depositor

Andrew Mesecar

Insert

hck (HCK Human)

Tags

6xHis-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Expression

Insect

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBacHTa_LYN

Plasmid

#224244

Purpose

Expression of human LYN Kinase in insect cells (SF9 cells)

Depositor

Andrew Mesecar

Insert

lyn (LYN Human)

Tags

6xHis-TEV

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-GFP-V5-His

Plasmid

#85621

Purpose

Expresses GFP and V5 in Drosophila.

Depositor

Kenneth Irvine

Article

Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

Insert

GFP

Tags

V5, His

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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46 pLIB_3C_C_HIS

Plasmid

#160188

Purpose

C-term 3C cleavable His tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pCFD3.1-w-dU6:3gRNA

Plasmid

#123366

Purpose

gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

Depositor

Simon Bullock

Insert

U6:3-gRNA

Use

CRISPR

Expression

Insect

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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