4,908 results

1. pJFRC81-3xVAS3-Syn21-V5-mCherry-P10

   Plasmid

   #104617

   Purpose

   Expresses V5 tagged mCherry upon binding of TALE3 to VAS3

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   Syn21-V5-mCherry-P10-MhcSA
   

   Use

   Tags

   V5

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. UAS-Halo7::CAAX

   Plasmid

   #87645

   Purpose

   For creating drosophila transgenics expressing UAS-Halo7::CAAX

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Halo7::CAAX
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBFP2

   Plasmid

   #165831

   Purpose

   EBFP2blue fluorescent protein CDS

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   EBFP2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1_GI.1_Q62C-A140C

   Plasmid

   #162581

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 62 changed to Cysteine and Alanine 140 …

   Promoter

   polyhedring

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

   Plasmid

   #217896

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB5 (PSMB5 Human)
   PSMB6 (PSMB6 Human)
   PSMB7 (PSMB7 Human)
   

   Use

   Tags

   2xSTII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACEBac1-PSMA5-PSMA6-PSMA7

   Plasmid

   #217894

   Purpose

   Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMA5 (PSMA5 Human)
   PSMA6 (PSMA6 Human)
   PSMA7 (PSMA7 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

   Plasmid

   #217897

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB1 (PSMB1 Human)
   PSMB2 (PSMB2 Human)
   PSMB3 (PSMB3 Human)
   PSMB4 (PSMB4 Human)
   

   Use

   Tags

   2xSTII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACEBac1-PSMB5-PSMB6-PSMB7

   Plasmid

   #217898

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB5 (PSMB5 Human)
   PSMB6 (PSMB6 Human)
   PSMB7 (PSMB7 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p4X-UASTattB

    Plasmid

    #189858

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-TRAF6-V5-His

    Plasmid

    #63752

    Purpose

    Expresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TRAF6 (Traf6 Fly)
    

    Use

    Tags

    V5-His

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)

    Plasmid

    #159553

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBFv-UAS3-GFP1-10

    Plasmid

    #138398

    Purpose

    Expresses split GFP fragment (GFP1-10)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFBOH-MHL_CORO1C:1-474

    Plasmid

    #210920

    Purpose

    Insect expression of full-length human CORO1C. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CORO1C:M1-A474 (CORO1C Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. AByG

    Plasmid

    #111083

    Purpose

    Integrates marker gene onto D. melanogaster Y chromosome

    Depositor

    Omar Akbari

    Article

    Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

    Inserts

    3xP3 promoter
    tdTomato
    Gypsy insulator
    CTCF insulator
    Y homology arm left
    Y homology arm right
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Use

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. SPHK1

    Plasmid

    #73260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-T2A-QF2-3xP3-RFP

    Plasmid

    #127559

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    QF2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge