Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Insect Expression Clear filter

Applied Filters

Insect Expression Clear filter

4,958 results

Showing: 301 - 350 of 4958 results

  2. pE6n

    Plasmid
    #17460
    Purpose
    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    EYFP
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17460-plasmid-map-sequence-id-8628

    Enlarge

  7. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

    Enlarge

  9. pCFD3-NS

    Plasmid
    #149545
    Purpose
    Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Promoter
    U6
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149545-plasmid-map-sequence-id-306357

    Enlarge

  15. LRP1A-c004

    Plasmid
    #205112
    Purpose
    LRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    LRP1 (LRP1 Human)
    Tags
    AviTag and GP64sp-6His-TEV
    Expression
    Insect
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205112-plasmid-map-sequence-id-412366

    Enlarge

  17. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

    Enlarge

  18. pAc5.1B-lambdaN-HA-HseIF4AI_T

    Plasmid
    #147543
    Purpose
    Insect Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Expression
    Insect
    Mutation
    3 silent mutations compared to the sequence given…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147543-plasmid-map-sequence-id-491297

    Enlarge

  29. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

    Enlarge

  33. pSR646

    Plasmid
    #65215
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-6 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available Since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65215-plasmid-map-sequence-id-112598

    Enlarge

  35. pExpreS2-1-C3-10H-SIII

    Plasmid
    #175444
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-10His-TwinStrep and BiP Secretion Peptide
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175444-plasmid-map-sequence-id-358135

    Enlarge

  38. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

    Enlarge

  39. pDR121

    Plasmid
    #37148
    Depositor
    Dale Ramsden
    Insert
    XRCC6 (XRCC6 Human)
    Tags
    H6
    Expression
    Insect
    Available Since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  43. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

    Enlarge

  46. pDR123

    Plasmid
    #37149
    Depositor
    Dale Ramsden
    Insert
    XRCC5 (XRCC5 Human)
    Expression
    Insect
    Available Since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  48. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 301 - 350 of 4958 results