78,292 results

1. pIRES-hrGFP II-TET1

   Plasmid

   #83568

   Purpose

   Expresses TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlpha12-RLuc8

   Plasmid

   #140985

   Purpose

   Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha12-RLuc8 (GNA12 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RN3P-KDM5B-FLAG

   Plasmid

   #86398

   Purpose

   FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

   Depositor

   Wei Xie

   Article

   Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

   Insert

   KDM5B (Kdm5b Mouse)
   

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   Lac

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5-AML1B

   Plasmid

   #12426

   Purpose

   pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

   Depositor

   Scott Hiebert

   Article

   Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

   Insert

   AML1B (RUNX1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSFFV_sfCherry2(1-10)

   Plasmid

   #82603

   Purpose

   Lentiviral vector for constitutive expression of sfCherry2(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   SFFV

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CKIIa-stGtACR1-FusionRed

   Plasmid

   #105679

   Purpose

   Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Insert

   stGtACR1
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Apple-53BP1trunc

   Plasmid

   #69531

   Purpose

   Fluorescent reporter for DNA damage

   Depositor

   Ralph Weissleder

   Article

   Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Lentiviral

   Tags

   Apple fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/TO/Strep-HA-ZAK beta

   Plasmid

   #141195

   Purpose

   Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 isoform 2 (MAP3K20 Human)
   

   Tags

   Strep, HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid

    #66790

    Purpose

    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRbeta (Pdgfrb Mouse)
    

    Tags

    C-term EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS-membrane-ceruleanFP

    Plasmid

    #53749

    Purpose

    Contains membrane localized cerulean fluorescent protein in pCS expression vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Tags

    Cerulean

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAP2K2-NanoLuc Fusion Vector

    Plasmid

    #238877

    Purpose

    Express MAP2K2-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

    Plasmid

    #236249

    Purpose

    Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV-CuO

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR p53

    Plasmid

    #12090

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    p53 shRNA (Trp53 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 HA-BACH1

    Plasmid

    #232269

    Purpose

    Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

    Plasmid

    #86885

    Purpose

    C-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    BirA* (AA141-321)- HA- NIPP1]
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 141-321

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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