78,292 results

1. plex_307-ccdB-g418

   Plasmid

   #201141

   Purpose

   pLEX_307 plasmid with g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RFP-ATF4

   Plasmid

   #125237

   Purpose

   ATF4 expression vector

   Depositor

   Rozita Yarmand

   Article

   Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

   Insert

   ATF4 (ATF4 Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HArcLight

   Plasmid

   #138326

   Purpose

   chemigenetic voltage indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Insert

   HArcLight
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

   Plasmid

   #90003

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-TFR-20

   Plasmid

   #55144

   Purpose

   Localization: Transferrin Receptor, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TFR (TFRC Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST53-LRRK2-G2019S

   Plasmid

   #25045

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Glycine 2019 mutated to Serine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

   Plasmid

   #187580

   Purpose

   Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NONO (NONO Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Orai1-GCaMP6f

   Plasmid

   #73564

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   Orai1-GCaMP6f (ORAI1 Human, Synthetic)
   

   Tags

   GCaMP6f

   Expression

   Mammalian

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2Flag-pDEST-C

    Plasmid

    #118372

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pf hPGK mc2 IRES puro

    Plasmid

    #206237

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAS-NanoLuc

    Plasmid

    #87696

    Purpose

    Gal4VP16 driven expression of NanoLuc

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NanoLuc
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT Smarca4-V5 IRES-puro

    Plasmid

    #107058

    Purpose

    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual-IN-SMN1

    Plasmid

    #215721

    Purpose

    dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN1 EXON 7 (SMN1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LRG2.1-TagBFP2

    Plasmid

    #124773

    Purpose

    Lentiviral plasmid to co-express a guide RNA and TagBFP2

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TRKB-V5/HIS

    Plasmid

    #205614

    Purpose

    Expression of human TRKB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TRKB receptor tyrosine kinase (NTRK2 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-flex-GFP-4x6T

    Plasmid

    #196418

    Purpose

    Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-Tol2 transposase

    Plasmid

    #158774

    Purpose

    Expression of Tol2 transposase

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    Tol2 tranposase
    

    Expression

    Mammalian

    Mutation

    Tol2 codon usage frequency well suitable for muri…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-RhoA-C1

    Plasmid

    #29674

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

    Insert

    RhoA (RHOA Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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