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78,616 results

Showing: 4771 - 4800 of 78616 results

  1. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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  3. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136456-plasmid-map-sequence-id-263271

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  9. GPRC5B-Tango

    Plasmid
    #66383
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPRC5B (GPRC5B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  19. RFP-(SUMO)6-(SIM)10

    Plasmid
    #122027
    Purpose
    Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122027-plasmid-map-sequence-id-434238

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  21. pLV-Golgi eGFP

    Plasmid
    #79809
    Purpose
    To express and target GFP into the Golgi apparatus using lentiviruses
    Depositor
    Pantelis Tsoulfas
    Insert
    Golgi eGFP (B4GALT1 Human)
    Use
    Lentiviral
    Tags
    Fusion of the first 62 amino acids of the human b…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    79809-plasmid-map-sequence-id-150299

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  27. TFORF2596

    Plasmid
    #142338
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RORB (RORB Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142338-plasmid-map-sequence-id-397178

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  30. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #84445
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84445-plasmid-map-sequence-id-177041

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Showing: 4771 - 4800 of 78616 results