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79,152 results

Showing: 3641 - 3660 of 79152 results

  2. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  11. DRD2-DuET

    Plasmid
    #213228
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD2 (DRD2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213228-plasmid-map-sequence-id-430789

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  15. TFORF2545

    Plasmid
    #144038
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPB (CEBPB Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144038-plasmid-map-sequence-id-392329

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  16. Kaede-C2

    Plasmid
    #54725
    Purpose
    Localization: C2 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Kaede
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54725-plasmid-map-sequence-id-482901

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  17. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid
    #204146
    Purpose
    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Article
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Inserts
    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    CMV and TRE3G
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204146-plasmid-map-sequence-id-404779

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Showing: 3641 - 3660 of 79152 results