Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Mammalian Expression Clear filter

Applied Filters

Mammalian Expression Clear filter

80,589 results

Showing: 8481 - 8520 of 80589 results

  5. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

    Enlarge

  6. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

    Enlarge

  8. TP53(Y220C)-HaloTag Fusion Vector

    Plasmid
    #236802
    Purpose
    Express TP53(Y220C)-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TP53(Y220C) (TP53 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    Y220C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236802-plasmid-map-sequence-id-471812

    Enlarge

  9. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid
    #206935
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206935-plasmid-map-sequence-id-407781

    Enlarge

  18. TFORF0985

    Plasmid
    #142249
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF4 (KLF4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    142249-plasmid-map-sequence-id-385602

    Enlarge

  19. m6A-Tracer-NES

    Plasmid
    #159607
    Purpose
    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.
    Depositor
    Aaron Streets
    Article
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Insert
    m6A-Tracer-NES
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159607-plasmid-map-sequence-id-311957

    Enlarge

  21. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid
    #141184
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has Service
    Cloning Grade DNA
    Insert
    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    Tags
    Signal peptide from influenza HA and Superfolder …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141184-plasmid-map-sequence-id-306284

    Enlarge

  22. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156912
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IGSF8 (IGSF8 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156912-plasmid-map-sequence-id-444413

    Enlarge

  24. pcDNA3.1(+) Drp1

    Plasmid
    #34706
    Depositor
    David Chan
    Insert
    Drp1 (Dnm1l Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    34706-plasmid-map-sequence-id-227765

    Enlarge

  27. TFORF1683

    Plasmid
    #142826
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TWIST1 (TWIST1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    142826-plasmid-map-sequence-id-392723

    Enlarge

  29. pcDNA3.1-cCFP

    Plasmid
    #185361
    Purpose
    For mammalian expression of C-term CFP for split-CFP
    Depositor
    Ayaz Najafov
    Insert
    CFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185361-plasmid-map-sequence-id-363642

    Enlarge

  31. pCDH-EF1-copGFP

    Plasmid
    #73030
    Purpose
    Express copGFP
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73030-plasmid-map-sequence-id-427120

    Enlarge

  32. mEmerald-FYN-N-10

    Plasmid
    #54099
    Purpose
    Localization: Membrane Associated Kinase, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FYN (FYN Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. NanoBRET Assay Vector, PPP2R1A-HaloTag

    Plasmid
    #238714
    Purpose
    Express PPP2R1A-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PPP2R1A (PPP2R1A Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238714-plasmid-map-sequence-id-479891

    Enlarge

  36. HiBiT-MRGPRX2 Fusion Vector

    Plasmid
    #238599
    Purpose
    Express HiBiT-MRGPRX2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MRGPRX2 (MRGPRX2 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238599-plasmid-map-sequence-id-479075

    Enlarge

Showing: 8481 - 8520 of 80589 results