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80,589 results

Showing: 10361 - 10400 of 80589 results

  12. pD649-HAsp-SIGLEC1(Trunc2)-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156999
    Purpose
    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC1 (SIGLEC1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156999-plasmid-map-sequence-id-447075

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  13. pD649-HAsp-SIGLEC6-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156762
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC6 (SIGLEC6 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156762-plasmid-map-sequence-id-447056

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  14. pD649-HAsp-MAG-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156882
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MAG (MAG Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156882-plasmid-map-sequence-id-446217

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  16. TFORF0444

    Plasmid
    #143701
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFX (ZFX Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143701-plasmid-map-sequence-id-471122

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  21. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  22. TFORF2364

    Plasmid
    #142051
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB1 (CREB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142051-plasmid-map-sequence-id-395673

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  23. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Expression
    Mammalian
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

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  28. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  36. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72259-plasmid-map-sequence-id-131837

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  37. pcDNA-GFP-STAT1

    Plasmid
    #11987
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    A188T, Q718R
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11987-plasmid-map-sequence-id-483719

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Showing: 10361 - 10400 of 80589 results