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82,463 results

Showing: 581 - 600 of 82463 results

  4. pcDNA-dCas9

    Plasmid
    #47106
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Flag and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47106-plasmid-map-sequence-id-433987

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  9. Lamp1-YFP

    Plasmid
    #1816
    Purpose
    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Publication
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available since
    Feb. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    1816-plasmid-map-sequence-id-364671

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  16. xCas9(3.7)-BE4

    Plasmid
    #108381
    Purpose
    Mammalian expression vector for xCas9(3.7)-BE4
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.7)-BE4
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    108381-plasmid-map-sequence-id-217618

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  19. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid
    #20945
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Insert
    channelrhodopsin-2
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    hSyn
    Available since
    May 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    20945-plasmid-map-sequence-id-442041

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Showing: 581 - 600 of 82463 results