81,074 results
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Plasmid#154088PurposeImproved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.DepositorUseLentiviralExpressionMammalianPromoterTet onAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only
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Nrf1-Myc pcDNA3.1(+)-C-Myc
Plasmid#169125Purposeexpression of proteinDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-WT
Plasmid#133749PurposeWild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimdDepositorInsertWild type EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianPromoterEF1-alphaAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-GPI-RUSH
Plasmid#166903PurposeRUSH cargo: Str-KDEL_IRES_SBP-Halo-GPIDepositorInsertHaloTag
ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HLA_A2402
Plasmid#165010PurposeExpression of HLA-A*24:02 cDNADepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-BLM
Plasmid#110299PurposeMammalian expression of a EGFP-tagged full length Bloom's syndrome helicaseDepositorAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagA-mCherry-Mito
Plasmid#162251PurposeBlue-light dependent heterodimerization systemDepositorInserteMagA-mCherry-OMP25
ExpressionMammalianPromoterbeta-actin promoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 GFPExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmExRai-AKAR2
Plasmid#161755PurposePlasma membrane-targeted ExRai-AKAR2.DepositorInsertLyn-ExRai-AKAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_mito Grx1-roGFP2
Plasmid#155044PurposeFluorescent redox reporterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseLentiviralTagsmitochondrial targeting sequence and roGFP2ExpressionMammalianPromoterCMVAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXG290
Plasmid#141284PurposeOE of HRIDepositorAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-HA.SV40(polyA)
Plasmid#154850PurposeExpresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminusDepositorAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha11-RLuc8
Plasmid#140983PurposeEncodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha11-RLuc8 (GNA11 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma8-GFP2
Plasmid#140990PurposeEncodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-GIGYF2
Plasmid#141189PurposeExpresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell lineDepositorAvailable SinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT FUCCI
Plasmid#136935Purposedonor plasmid for cardiac-specific FUCCI expression in human cellsDepositorInsertTroponin T promoter
UseSynthetic BiologyExpressionMammalianPromotercTnTAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plasma membrane-targeted optoPLD
Plasmid#140114PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expressionDepositorInsertPhospholipase D
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP2-C-Flag
Plasmid#134287PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP2 precursorPromoterCMVAvailable SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABE8e
Plasmid#138493PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (CP1041)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-TOM20_Halo_T2A_EGFP
Plasmid#135443PurposeMamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFPDepositorInsertTOM20-HaloTag7 and EGFP
TagsTOM20ExpressionMammalianPromoterCMV/TetOAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmNeonGreen
Plasmid#129606PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorInsertF-tractin
ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Rheb
Plasmid#133767PurposeExpresses GFP-tagged Rheb in mammalian cellsDepositorAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only