78,128 results

1. c-myc promoter (Del 1)

   Plasmid

   #16601

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 1) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-1 (XmnI->PvuII)

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-SADB19L

   Plasmid

   #32632

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   SADB19L
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. Beta-2-adrenergic receptor-CFP

   Plasmid

   #55794

   Purpose

   Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Beta-2-adrenergic receptor-ECFP (ADRB2 Human, Aequorea victoria)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. cmv-mCherry-10xPP7

   Plasmid

   #185796

   Purpose

   mCherry labeled with 10xPP7

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-mCherry-10xPP7
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1389 IBB-GFP-mCherry3E

   Plasmid

   #118803

   Purpose

   Fluorescence-based TDP43-dependent splicing reporter

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   Synthetic
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SuExp His-Myc-hPLD4

   Plasmid

   #173852

   Purpose

   Protein expression plasmid for recombinant His-Myc tag human PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (PLD4 Human)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFGAMS-mEos2

   Plasmid

   #105128

   Purpose

   Expresses human FGAMS-mEos2 fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   French et al Science. 2016 Feb 12;351(6274):733-7. do

   Insert

   PFAS (PFAS Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EBFP2-Cx30

    Plasmid

    #69022

    Purpose

    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BIC-Gag-CAS9

    Plasmid

    #119942

    Purpose

    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

    Depositor

    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG FMLV fused to spCAS9
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    hCMV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

    Plasmid

    #139308

    Purpose

    N-terminal half of split PI-PLC targeted to plasma membrane

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
    

    Expression

    Mammalian

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

    Plasmid

    #89609

    Purpose

    over-expression of VEGF-A(165) in human cells

    Depositor

    Fernando Fierro

    Insert

    VEGF-A(165) (VEGFA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K188E

    Promoter

    MNDU3

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid

    #139992

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. negAKARet-cyto

    Plasmid

    #114733

    Purpose

    Negative control of AKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of AKARet-cyto
    

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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