80,773 results

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Lenti-LucOS

Plasmid

#22777

Depositor

Tyler Jacks

Insert

OVA-SIY

Use

Cre/Lox and Lentiviral

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_C_AOX

Plasmid

#111661

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AOX

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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p52sheLL

Plasmid

#46950

Purpose

HPV52 L1 and L2 Bicistronic, too large to self-package

Depositor

John Schiller

Article

Kondo et al J Med Virol. 2008 May;80(5):841-6. doi:

Insert

HPV52L1+L2 (L1, L2 Human)

Expression

Mammalian

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MEF2C-HA

Plasmid

#32515

Depositor

Andrew Lassar

Article

Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

Insert

Mef2c (Mef2c Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

Plasmid

#103870

Purpose

High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GSGGGGS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCI H2B-RFP

Plasmid

#92398

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

IRES H2B RFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-15:ACTB-mEGFP

Plasmid

#87425

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

ACTB Homology Arms with mEGFP-linker (ACTB Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pB80-2xFKBP-L-mCherry-CAAX

Plasmid

#193713

Purpose

Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

2xFKBP-L-mCherry-CAAX

Tags

2xFKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_STA5B

Plasmid

#167800

Purpose

Expresses STA5B in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5B (STAT5B Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_TetOn_Hu_BIRC5(WT)

Plasmid

#136348

Purpose

Lentiviral inducible expression (TetOn) of human BIRC5

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCI-neo-His-MS2BP-G3BP1-WT

Plasmid

#136008

Purpose

WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

MS2BP

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2

Plasmid

#237637

Purpose

For overexpression of mEGFP-SS18-SSX2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2 (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-gp78-FLAG

Plasmid

#62370

Purpose

Express C-terminally FLAG tagged gp78

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

gp78 (AMFR Human)

Tags

FLAG

Expression

Mammalian

Mutation

*

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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LP538

Plasmid

#90202

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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