78,128 results

1. TFORF0723

   Plasmid

   #142226

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL4393 (pClpX_Eco)

   Plasmid

   #200907

   Purpose

   Expresses human codon-optimized E. coli ClpX from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   EcoClpX
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABE8r

   Plasmid

   #208294

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1.3xWT HBV-Luciferase

   Plasmid

   #61549

   Purpose

   The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core

   Depositor

   Yosef Shaul

   Article

   Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.

   Insert

   1.3 times of the HBV genome with luciferase within the Core ORF
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Substitution of HBV sequences with luciferase ORF…

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

   Plasmid

   #216244

   Purpose

   Express AtAFB2.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A-mCherry
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1 human cofilin S3A

   Plasmid

   #50860

   Purpose

   expresses constituvely active cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3A constitutively active

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRosa26-DEST

   Plasmid

   #21189

   Purpose

   Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

   Depositor

   Nick Hastie, Peter Hohenstein

   Article

   Hohenstein et al Pathogenetics. 2008 . 1(1):3.

   Insert

   GateWay conversion cassette
   

   Expression

   Mammalian

   Available Since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. gag-TadCBEd

   Plasmid

   #225144

   Purpose

   Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-TadCBEd
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN7-S15

    Plasmid

    #190665

    Purpose

    Expresses N7 ribosomal protein S15

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7 S15
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTq2-Calmodulin

    Plasmid

    #198200

    Purpose

    FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    Calmodulin 2 (Calm2 Rat)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA-3xFLAG-Pontin

    Plasmid

    #51635

    Purpose

    Mammalian expression of 3xFLAG tagged pontin, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    Pontin (RUVBL1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-myc-CXCR4

    Plasmid

    #98946

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3XFLAG-TAK1-K63A

    Plasmid

    #43933

    Depositor

    Bryant Darnay

    Article

    Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

    Insert

    Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    K63A

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ApaLID211A-HA

    Plasmid

    #211459

    Purpose

    Express mitochondrial localized ApaLI with D211A mutation

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-D211A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D211A

    Promoter

    CMV

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Tph2

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_MCP-p300_Hygro

    Plasmid

    #138459

    Purpose

    lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLXRN-EGFP

    Plasmid

    #99207

    Purpose

    EGFP expressing bicistronic retroviral vector

    Depositor

    Peter Pedersen

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A Kozak sequence was incorporated to enhance tran…

    Promoter

    LTR

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Gαi*-BERKY3

    Plasmid

    #158198

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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