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pAAV-CAG-FlpX-mKate2.0-N2cG

Plasmid

#234711

Purpose

pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

mKate2.0 T2A N2cG

Use

AAV

Tags

T2A

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-CDDR

Plasmid

#149355

Purpose

CRISPR/Cas9-based dual-fluorescent DSB repair reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

CDDR Reporter
Blasticidin resistance

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a and hPGK

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-HBXIP

Plasmid

#42326

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMY-mCherry-P2A-PuroR-HA

Plasmid

#163342

Purpose

Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-SP110C

Plasmid

#65393

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110C

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pTT3 Flrt2-myc

Plasmid

#72192

Purpose

Express full-length Flrt2with a C-terminal myc tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt2 (Flrt2 Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHS2 (hANGPTL4-V5)

Plasmid

#102446

Purpose

Expresses V5-tagged human ANGPTL4 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

ANGPTL4 (ANGPTL4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcmCherry 3.1(-)

Plasmid

#62803

Purpose

Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry

Depositor

Michal Minczuk

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Kl-EGFP

Plasmid

#45532

Depositor

Zipora Yablonka-Reuveni

Article

Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

Insert

alpha Klotho (Kl Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-PCAF (delta5-53a.a.)

Plasmid

#65388

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PCAF (KAT2B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

lost 13-159bp of the ORF

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pGMC00009 (aka pRC0494)

Plasmid

#195294

Purpose

Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP

Depositor

Raj Chari

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1.1

Plasmid

#208026

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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tetO-TBX20

Plasmid

#170705

Purpose

doxycycline-inducible overexpression of TBX20

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TBX20 (TBX20 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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