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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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82,530 results

Showing: 10141 - 10160 of 82530 results

  1. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196061-plasmid-map-sequence-id-385874

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  7. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Publication
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Expression
    Mammalian
    Promoter
    RNA polymease I Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

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  9. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

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  13. pT3-EF1aH

    Plasmid
    #180149
    Purpose
    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.
    Depositor
    Xin Chen
    Publication
    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF1α
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180149-plasmid-map-sequence-id-357865

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  16. AAV-CAG-jGCaMP8m-WPRE

    Plasmid
    #179255
    Purpose
    AAV-mediated expression of GCaMP8m under the CAG promoter
    Depositor
    Loren Looger
    Has service
    AAV1, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179255-plasmid-map-sequence-id-354032

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  20. pcDNA3-EKAR4

    Plasmid
    #174437
    Purpose
    Improved cyan/yellow FRET-based ERK kinase activity reporter.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174437-plasmid-map-sequence-id-492002

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Showing: 10141 - 10160 of 82530 results