Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Mammalian Expression Clear filter

Applied Filters

Clear All Filters
Mammalian Expression Clear filter

82,546 results

Showing: 10701 - 10720 of 82546 results

  3. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

    Plasmid
    #163844
    Purpose
    Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Tags
    mKate2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    163844-plasmid-map-sequence-id-325978

    Enlarge

  11. pCAGGS-SARS2-S-D614 (C-flag)

    Plasmid
    #156420
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    156420-plasmid-map-sequence-id-307822

    Enlarge

  12. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

    Enlarge

  13. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

    Enlarge

  14. pcDNA3.1-Beta1

    Plasmid
    #140987
    Purpose
    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBeta1 (GNB1 Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal human rhinovirus (HRV) 3C …
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140987-plasmid-map-sequence-id-301588

    Enlarge

  19. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Publication
    Yang et al PLoS One. 2026 Jul 23;21(7):e0352468. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135049-plasmid-map-sequence-id-269408

    Enlarge

Showing: 10701 - 10720 of 82546 results