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Mammalian Expression

75,750 results

Showing: 3401 - 3440 of 75750 results

  5. pAAV-hSyn-hChR2(H134R)-EYFP

    Plasmid
    #26973
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26973-plasmid-map-sequence-id-199313

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  7. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  8. pCLIP-tag (m) Vector

    Plasmid
    #101136
    Purpose
    Expression of protein of interest with CLIP-Tag in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Type
    Empty backbone
    Use
    Tags
    CLIP-tag (CLIP10)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101136-plasmid-map-sequence-id-194177

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  10. MSCV Luciferase PGK-hygro

    Plasmid
    #18782
    Depositor
    Scott Lowe
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18782-plasmid-map-sequence-id-106035

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  12. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  15. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Use
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  21. TFORF0172

    Plasmid
    #142551
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT2 (ONECUT2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    142551-plasmid-map-sequence-id-397438

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  27. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Article
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231580-plasmid-map-sequence-id-462114

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  28. pcDNA3-ExRai-AktAR2

    Plasmid
    #184047
    Purpose
    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    ExRai-AktAR2
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184047-plasmid-map-sequence-id-363388

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  33. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid
    #155045
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155045-plasmid-map-sequence-id-310403

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  34. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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  38. pcDNA3-TLR7-YFP

    Plasmid
    #13022
    Depositor
    Doug Golenbock
    Insert
    TLR7 (TLR7 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13022-plasmid-map-sequence-id-476228

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  40. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VC155
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22011-plasmid-map-sequence-id-354492

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Showing: 3401 - 3440 of 75750 results