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79,901 results

Showing: 1821 - 1840 of 79901 results

  2. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

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  10. pD649-HAsp-LILRB2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156863
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LILRB2 (LILRB2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    156863-plasmid-map-sequence-id-382983

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  12. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  14. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Article
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Insert
    UTX (KDM6A Human)
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available Since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 1821 - 1840 of 79901 results