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SPLICS PM-ER Long P2A

Plasmid

#164111

Purpose

Detect the long Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

Plasmid

#171383

Purpose

Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized minidCas13X.1-REPAIRv2

Tags

HA

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAP

Plasmid

#166457

Purpose

Expresses fusion of mEGFP and YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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ALDH18A1

Plasmid

#155402

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ALDH18A1 (ALDH18A1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GPRC5B-Tango

Plasmid

#66383

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPRC5B (GPRC5B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat, Budding Yeast)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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IronFist

Plasmid

#243006

Purpose

Genetically encoded fluorescent iron reporter

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

Hemerythrin-like (FBXL5 Human)
T2A
mCherry

Use

Gateway

Tags

mNeonGreen

Expression

Mammalian

Promoter

human phosphoglycerate kinase (hPGK)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 wt

Plasmid

#107104

Purpose

Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Caveolin-C-10

Plasmid

#55008

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS2

Plasmid

#185137

Purpose

non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Neo DEST (685-3)

Plasmid

#17292

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GAL4

Plasmid

#24345

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLD-puro-MTS-EGFP

Plasmid

#190270

Purpose

Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

Depositor

Christian Münch

Article

Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

Insert

MTS-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-IIΔ

Plasmid

#35176

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the entire C-terminal domain (repeats…

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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