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Plasmid#136133PurposePlasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:mTurq-N7
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB516
Plasmid#74858PurposeGateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZY101
Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWBs00
Plasmid#222476PurposeMarchantia polymorpha transformation vector carrying sulfadiazine selection marker.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (noNLS) nog
Plasmid#119554PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p16 nlsGPS1
Plasmid#101081PurposeGA biosensor expression plasmidDepositorInsertnlsGPS1
UseTagscMycExpressionPlantMutationPromoterp16 (AT3G60245)Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-nlsSED1
Plasmid#178190PurposeSED1 biosensor for expression in Arabidopsis thalianaDepositorInsertSED1 biosensor
UseTagsSV40 NLSExpressionPlantMutationPromoterpUBQ10Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB203
Plasmid#68594PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEL853
Plasmid#214253PurposeExpression TadDE in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9569
Plasmid#219431PurposeTranscriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOSDepositorInsertLB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsPapE-4LF2ExpressionPlantMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB201
Plasmid#68592PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM134
Plasmid#127215PurposeBeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDSDepositorInsertLuc, IPT, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PROM5_MpEF1a
Plasmid#136113PurposeStrong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notchesDepositorInsertPROM5_MpEF1a Mapoly0024s0116
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)
Plasmid#160607PurposeTU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.DepositorInsertCUP2Gal4AD
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AL12R-HSP101
Plasmid#161014PurposePP7-tagged reporter driven by the Arabidopsis HSP101 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE4
Plasmid#195369PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInserthA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D4910
Plasmid#127220PurposeModule D with ZmUbi1::BBMDepositorInsertBBM
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265_OoA3GX2
Plasmid#225147PurposeGateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editingDepositorInsertOoA3GX2-zCas9(D10A)-UGI
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIKU801-CrPEPC1
Plasmid#92342PurposeExpresses sgRNA for targeting CrPEPC1 gene, paromomycin resistanceDepositorInsertsgRNA-CrPEPC1
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJB296
Plasmid#107691Purpose35SPromoter-LwaCas13a-HSPTerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB204
Plasmid#68595PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE5
Plasmid#195371PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8DepositorInsertecTadA8e-(GGGGS)x8-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UtB2N7
Plasmid#161015Purposeubiquitously expressed nuclear localized tagBFP2DepositorInserttagBFP2
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOE11 Lat52:mCherry transient
Plasmid#124249PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCk2
Plasmid#136696PurposeAcceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)
Plasmid#191170PurposeEK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.DepositorInsertmCherry:s70587:EGFP (plant)
UseTagsExpressionPlantMutationWTPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only