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Plasmid#161005Purposeubiquitous expression of MCP-sfGFPDepositorInsertMCP-sfGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE083
Plasmid#149279PurposeT-DNA encoding TRV2 complete genome with MCSDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p35S-A3A-PBE
Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW646 - pML1>>
Plasmid#115995Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH-roGFP2-ORP1
Plasmid#191677PurposeTi plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.DepositorInsertredox(ro)GFP2-ORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW-GFP-GOI-TurboID
Plasmid#209382PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/eGFP-C
Plasmid#183152PurposePlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10
Plasmid#197952PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspcoCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26 and pUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9-@GL1
Plasmid#86779PurposeDisruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9DepositorInsertsgRNA against GLABROUS1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterArabidopsis U6-26 promoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZG61
Plasmid#202575PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)
Plasmid#160634PurposeTU for wtGFP expression driven by 2x35s promoter.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter2x35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS_mALS
Plasmid#136081PurposeConfers plant resistance to chlorsulfuronDepositorInsertCDS_mALS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1_KanR-Ck1
Plasmid#136123PurposeL1 in position 1 (next to LB in L2 construct), for KanRDepositorInsertp5-MpEF1a:nptII_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-synORP1
Plasmid#191701PurposeTi plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.DepositorInsertchl-roGFP2-synORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_nCas9-PmCDA_Hyg_ALS
Plasmid#91693PurposeMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL11137
Plasmid#191783PurposeKanamycin resistance in plantsDepositorInsert35S::nptII-T-Nos
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-synORP1
Plasmid#191759PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.DepositorInsertnuc-roGFP2-synORP1
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW648 - pREV>>
Plasmid#115996Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)
Plasmid#160627PurposeTU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-MS2:TV-Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB420
Plasmid#74814PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRVQ
Plasmid#200188PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editingDepositorInsertLbCas12a-RRVQ
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182V, E795QPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3-eIFiso4E120(+)
Plasmid#86092PurposeThis vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.DepositorInserteIFiso4E
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-03
Plasmid#125762PurposeEndlinker for tRNA-gRNA system when using only three guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
Plasmid#115487PurposeCRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSE901
Plasmid#91709PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_231
Plasmid#91120PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ281 (MbCas12a)
Plasmid#138113PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoterDepositorInsertMbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only