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4,545 results

Showing: 1701 - 1750 of 4545 results

  2. pYPQ284-v2

    Plasmid
    #138119
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a v2
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D172R, N563R and K569R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138119-plasmid-map-sequence-id-272076

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  3. pVL47 - pSCR>>

    Plasmid
    #116013
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Thomas Greb
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    116013-plasmid-map-sequence-id-228504

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  6. STEME-1

    Plasmid
    #138137
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    Expression
    Plant
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    138137-plasmid-map-sequence-id-320280

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  9. pUPD1:Rb7-MAR

    Plasmid
    #106212
    Purpose
    Provides N.tabacum RB7 matrix attachment region as a level 0 GoldenBraid part
    Depositor
    Tomáš Moravec
    Insert
    N.tabacum RB7 matrix attachment region
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106212-plasmid-map-sequence-id-205304

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  10. INCTbiosyn-p35S-GFP(rc)13

    Plasmid
    #127526
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127526-plasmid-map-sequence-id-250841

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  13. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

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  20. pDIRECT_23D

    Plasmid
    #91141
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91141-plasmid-map-sequence-id-182269

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  23. pYPQ269C2

    Plasmid
    #164716
    Purpose
    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible haid-cbe_v04 entry clo…
    Expression
    Plant
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164716-plasmid-map-sequence-id-325386

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  38. pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)

    Plasmid
    #160642
    Purpose
    Module for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    GRLacIBDGal4AD / RDF
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160642-plasmid-map-sequence-id-321667

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  41. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid
    #75409
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [D1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75409-plasmid-map-sequence-id-211946

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  43. pUPD1:TM2-MAR

    Plasmid
    #106214
    Purpose
    Provides N.tabacum TM2 matrix attachment region as a level 0 GoldenBraid part
    Depositor
    Tomáš Moravec
    Insert
    N.tabacum TM2 matrix attachment region
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106214-plasmid-map-sequence-id-205308

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  48. pYPQ262m

    Plasmid
    #161522
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA(wt)-TadA(7.10)-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…
    Expression
    Plant
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161522-plasmid-map-sequence-id-325551

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Showing: 1701 - 1750 of 4545 results