3,991 results

1. TF101-STTM-miR165/166

   Plasmid

   #84816

   Purpose

   Turning off/down miR165/166 in Soybean

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM-miR165/166
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   unknown

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pFGC5941-PacI-ath-STTM829.2

   Plasmid

   #84158

   Purpose

   target miRNA829.2 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM829.2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB216

   Plasmid

   #68607

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Nuclear localization signal-tagged tdTomato (tdTo…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMDC43-SEIPIN2

   Plasmid

   #96977

   Purpose

   Express GFP-tagged Arabidopsis SEIPIN2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN2 (AT1G29760 Mustard Weed)
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMDC43-SEIPIN1

   Plasmid

   #96976

   Purpose

   Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN1 (AT5G16460 Mustard Weed)
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. UBQ10:sXVE:ER-GFP1–10

   Plasmid

   #125664

   Purpose

   Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Methods Mol Biol. 2021;2200:323-336. doi

   Insert

   ER-GFP1–10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBQ10:sXVE:

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCO489

   Plasmid

   #139732

   Purpose

   Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pMOD_D_AtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtuMAS

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGWB423

   Plasmid

   #68688

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Triple repeats of Citrine (3xCitrine)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAtCas9-dead_1

   Plasmid

   #91165

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPOR2KN0040

    Plasmid

    #117607

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTX208

    Plasmid

    #89268

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsYSA-gRNA01 and OsYSA-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. tRNA-gRNA position [2_n-1] (GB1206)

    Plasmid

    #75407

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [2_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEELA

    Plasmid

    #79752

    Purpose

    binary DEST vector for plant expression

    Depositor

    Nico Dissmeyer, Marc Jakoby

    Article

    Jakoby et al FEBS Lett. 2004 Nov 19;577(3):528-34.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2xPro35S

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MIGS4.2 (FF575)

    Plasmid

    #35252

    Depositor

    Detlef Weigel

    Article

    de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR1CB0058

    Plasmid

    #117539

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (SaCas9)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZA71

    Plasmid

    #174785

    Purpose

    In planta gene expression

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
    

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZA68

    Plasmid

    #174781

    Purpose

    In planta gene expression

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker
    

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    L17E/D481V

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZA66

    Plasmid

    #174779

    Purpose

    In planta gene expression

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker
    

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    D481V

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZA65

    Plasmid

    #174778

    Purpose

    In planta gene expression

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-eGFP, Kmr plant selection marker
    

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZA67

    Plasmid

    #174780

    Purpose

    In planta gene expression

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker
    

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    L17E

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid

    #160635

    Purpose

    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRATIO2251

    Plasmid

    #141305

    Purpose

    Gateway cloning compatible ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    Firefly Luciferase or LUC2 and mScarlet-I

    Expression

    Plant

    Mutation

    Promoter

    UBQ10 promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMpGWB106-OEP7(1–50)

    Plasmid

    #100602

    Purpose

    Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50)-Citrine (OEP7 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 35S-frGFP-nosT

    Plasmid

    #80128

    Purpose

    Transient expression vector for frGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Use

    Tags

    frGFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid

    #115489

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSC50

    Plasmid

    #104824

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.08g081600, Glyma.05g126600
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

    Plasmid

    #160641

    Purpose

    Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJG493

    Plasmid

    #91197

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gUbi1+gUbi8+donor
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pG20_LUC2_Hyg

    Plasmid

    #159707

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    luciferase

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pG20_GUS_Blp

    Plasmid

    #159706

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge