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pJW1586

Plasmid

#121057

Purpose

mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mKate2-T^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized mKate2, and au…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBN41

Plasmid

#86716

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-2::GFP

Expression

Worm

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Publication

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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lis-1::L4440

Plasmid

#11336

Depositor

Guy Caldwell

Insert

lis-1 (lis-1 Nematode)

Use

RNAi

Expression

Worm

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
L4816

Plasmid

#1682

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, gfpN, IVSR, gfpY66W, IVSS, gf…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSKI

Plasmid

#232484

Purpose

pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

Depositor

Carlos Silva García

Publication

Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

Insert

pSKI

Expression

Worm

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Publication

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBN42

Plasmid

#86713

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-3::GFP

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Publication

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Tags

GFP and HIS

Expression

Worm

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Publication

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Publication

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Publication

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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TU#712

Plasmid

#15280

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzyfp

Expression

Worm

Mutation

S65G, V68L, S72A, and T203Y

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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L3787

Plasmid

#1594

Depositor

Andrew Fire

Type

Empty backbone

Tags

hsp16-415', hsp16-25', T7p, gfpN, IVSR,…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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