1,782 results

Showing: 331 - 360 of 1782 results

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pCFJ150-GFP(dpiRNA)::CDK-1

Plasmid

#107938

Purpose

cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)

Expression

Worm

Promoter

cdk-1

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1-rab3P::his-24::mNeptune

Plasmid

#124350

Purpose

Nuclear Localized Pan-Neuronal mNeptune

Depositor

Andrew Leifer

Insert

rab-3, mNeptune (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM271 - MCS mosTI NeoR target

Plasmid

#159830

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI NeoR target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1320

Plasmid

#84829

Purpose

Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized GFP

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

Plasmid

#66753

Purpose

miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Worm and Yeast

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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BSP610

Plasmid

#122255

Purpose

expression vector for mtagBFP2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagBFP2

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pXF88

Plasmid

#139039

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (includes STOP codon, PATC introns)

Expression

Worm

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDD121

Plasmid

#91833

Purpose

Cas9 expression in C. elegans

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Cas9

Use

CRISPR

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAV1825

Plasmid

#37838

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2DsRed::KDEL

Tags

KDEL

Expression

Worm

Promoter

nhx-2

Available Since

Dec. 5, 2012

Availability

Academic Institutions and Nonprofits only
pMB70

Plasmid

#47943

Purpose

Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

guide RNA

Use

CRISPR

Expression

Worm

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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XW09

Plasmid

#65833

Purpose

Punc-4c-QS-SL2-mcherry3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Expression

Worm

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3814

Plasmid

#154122

Purpose

mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::rtetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBN1

Plasmid

#86710

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Insert

Plmn-1::mCherry::his-58::pie-1 3'UTR

Expression

Worm

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

Plasmid

#107132

Purpose

cGAL-N driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

Plasmid

#107136

Purpose

cGAL-C driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPD49_83

Plasmid

#1448

Depositor

Andrew Fire

Type

Empty backbone

Tags

hsp16-25', hsp16-415', IVSA, rf2128

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJA281

Plasmid

#22513

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

Pmex-5::mCherry (w introns w/o stop)

Expression

Worm

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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1,782 results