1,779 results

1. pBN396

   Plasmid

   #109326

   Purpose

   Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

   Depositor

   Peter Askjaer

   Article

   Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

   Insert

   mex-5 (mex-5 Nematode)
   

   Expression

   Worm

   Promoter

   mex-5

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

   Plasmid

   #182351

   Purpose

   Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

   Plasmid

   #66753

   Purpose

   miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

   Depositor

   Yishi Jin

   Article

   Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   miniSOG
   

   Tags

   tomm-20 (N terminal 55 aa)

   Expression

   Worm

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1839

   Plasmid

   #154325

   Purpose

   sgRNA Destination Vector for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR021

   Plasmid

   #198814

   Purpose

   Variant of yellow fluorescent protein for transient gene expression

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::destabilizedYFP::let-838 3'UTR
   

   Expression

   Worm

   Mutation

   YFP with destabilized signal from mouse ornithine…

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHW450

   Plasmid

   #198831

   Purpose

   Voltage-sensitive fluorescent protein.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD378

   Plasmid

   #91827

   Purpose

   Codon-optimized HaloTag donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   HaloTag-C1
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159813

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRB1080

    Plasmid

    #71309

    Purpose

    Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    VQR Cas9 variant
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS235

    Plasmid

    #191855

    Purpose

    Negative selection marker for MosSCI

    Depositor

    K Subramaniam

    Article

    Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

    Insert

    peel-1 toxic protein for negative selection against extrachromosomal array
    

    Expression

    Worm

    Promoter

    hsp-16.41

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF11-wemiRFP670-T2A-HO1

    Plasmid

    #197243

    Purpose

    Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wemiRFP670-T2A-HO1
    

    Expression

    Worm

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. XW09

    Plasmid

    #65833

    Purpose

    Punc-4c-QS-SL2-mcherry3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Neurospora crassa OR74A repressor (qa-1S)
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Punc-4c

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB16 (pmyo-3::QuasAr)

    Plasmid

    #130272

    Purpose

    Expresses the voltage sensor QuasAr in BWMs of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr
    

    Expression

    Worm

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS2301

    Plasmid

    #37834

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2cpl-1::YFP (cpl-1 Nematode)
    

    Tags

    YFP

    Expression

    Worm

    Promoter

    nhx-2

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW2341

    Plasmid

    #186950

    Purpose

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

    Depositor

    Jordan Ward

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMS050

    Plasmid

    #91826

    Purpose

    Codon-optimized mScarlet-I donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mScarlet-I-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJA281

    Plasmid

    #22513

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    Pmex-5::mCherry (w introns w/o stop)
    

    Expression

    Worm

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHZ081

    Plasmid

    #27506

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Tags

    BFP

    Expression

    Worm

    Promoter

    Plgc-55B

    Available Since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Expression

    Worm

    Promoter

    None

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJH127

    Plasmid

    #162483

    Purpose

    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. Pvha6-AgDD

    Plasmid

    #80626

    Purpose

    create protein aggregates in C. elegans

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Tags

    MLALKLAGLDI

    Expression

    Worm

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge