1,755 results

1. Pvha6-AgDD

   Plasmid

   #80626

   Purpose

   create protein aggregates in C. elegans

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

   Plasmid

   #107133

   Purpose

   cGAL-N driver under the control of C. elegans hsp-16.41 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::cGAL(DBD)::gp41-1-N-intein
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans hsp-16.41 promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN444

   Plasmid

   #108331

   Purpose

   Expresses Sat2 for Nourseothricin resistance selection of C. elegans

   Depositor

   Shinsuke Niwa

   Article

   Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

   Insert

   Streptothricin acetyl transferase 2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-27 promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

   Plasmid

   #159799

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBN41

   Plasmid

   #86716

   Purpose

   C. elegans injection marker

   Depositor

   Peter Askjaer

   Article

   Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

   Insert

   Pmyo-2::GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDD288

   Plasmid

   #137017

   Purpose

   mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mNG-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mNG

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xHA-BioID_pAS28

   Plasmid

   #118223

   Purpose

   expresses 3xHA-tagged BioID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   BioID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   R118G

   Promoter

   ges-1p

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY51

   Plasmid

   #191054

   Purpose

   mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   mec-3 (mec-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEY26

   Plasmid

   #191031

   Purpose

   cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.3-2.6k) (cho-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTK73.ChrII_ttTi5605.sgRNA

    Plasmid

    #194058

    Purpose

    sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrII_ttTi5605 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTK73.ChrV_oxTi365.gRNA

    Plasmid

    #194052

    Purpose

    sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrV_oxTi365 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TC476

    Plasmid

    #122629

    Purpose

    Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SP6-sgRNA-scaffold

    Plasmid

    #47912

    Purpose

    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    sgRNA 3' end

    Expression

    Worm

    Mutation

    Promoter

    SP6

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSDH61

    Plasmid

    #139033

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (no STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEY52

    Plasmid

    #191055

    Purpose

    odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    odr-1 (odr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA304

    Plasmid

    #28091

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ1662

    Plasmid

    #51482

    Purpose

    miniMos transposable element with HygroR, MCS cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWP018

    Plasmid

    #198766

    Purpose

    Drives specific expression in the ADE, CEP, and PDE neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pdat-1-GAL4-SK(DBD)-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BSP188

    Plasmid

    #110917

    Purpose

    For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    unc54 Terminator
    

    Use

    Synthetic Biology; Yeast homologous recombination…

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFJ1663

    Plasmid

    #51484

    Purpose

    miniMos transposable element with HygroR and peel-1, MCS cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSM03

    Plasmid

    #139043

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    dendra2 (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNM3732

    Plasmid

    #153086

    Purpose

    mec-4p GFP-C1 positive control for RMCE integration

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p GFP-C1 tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

    Plasmid

    #130275

    Purpose

    Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    Arch(D95N)
    

    Use

    Tags

    2x Myc-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMLS485

    Plasmid

    #188890

    Purpose

    Worm Cas9 expression vector, Peft-3 promoter

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Peft-3::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ210

    Plasmid

    #30538

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCFJ1655

    Plasmid

    #51481

    Purpose

    miniMos transposable element with HygroR, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge