1,755 results

1. pEft-3::oxCerulean-oxVenus::Lgg-1

   Plasmid

   #68040

   Purpose

   Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagy

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::oxCerulean-oxVenus::Lgg-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. punc-119cR

   Plasmid

   #21596

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119:mCherry (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   mCherry

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH3377

   Plasmid

   #175286

   Purpose

   Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   daf-16 d/f (daf-16 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pges-1

   Available since

   Nov. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

   Plasmid

   #129538

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. XW52

   Plasmid

   #65838

   Purpose

   XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   Czipper-QF-AD-NLS
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Pmig-13

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168170

   Purpose

   Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   E497K and C566D

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

   Plasmid

   #108362

   Purpose

   Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

   Depositor

   Luisa Cochella

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Inserts

   Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
   mCherry
   his-15 (his-15 Nematode)
   unc-54 3' UTR
   

   Use

   Tags

   2xFlag, MYC, T2A peptide, and TY1 linker

   Expression

   Worm

   Mutation

   Promoter

   A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEY12

    Plasmid

    #191018

    Purpose

    Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    rab-3 (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD_Psra-6::ChR2(H134R)::mCherry

    Plasmid

    #117419

    Purpose

    For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    sra-6

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEY46

    Plasmid

    #191050

    Purpose

    glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    glr-1 (glr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEY38

    Plasmid

    #191042

    Purpose

    unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    unc-8 (unc-8 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEY13

    Plasmid

    #191019

    Purpose

    Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    arrd-4 (ZK938.2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEY20

    Plasmid

    #191026

    Purpose

    gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gcy-18 (gcy-18 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMLS328

    Plasmid

    #73717

    Purpose

    C. elegans germline CRE expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-CRE
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST- IP2.0(codon for C.elegans)

    Plasmid

    #109216

    Purpose

    IP2.0 for C. elegans (Codon optimization and introns).

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDR3.10

    Plasmid

    #19367

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    msp-142 ORF+3' in pDONR201
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

    Plasmid

    #159896

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMLS286

    Plasmid

    #73723

    Purpose

    Saptrap tagRFP + Cbr-unc-119 donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    tagRFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSR02

    Plasmid

    #69149

    Purpose

    Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPD176.36

    Plasmid

    #1720

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    T7p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacIN…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJH4.52

    Plasmid

    #26946

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    histone H2B (his-58 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGM48DEST

    Plasmid

    #74757

    Purpose

    Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

    Depositor

    Keith Yamamoto, Jordan Ward

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Inserts

    QS
    Gateway cassette
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge