1,779 results

1. pMNK56 - piRNAi 21ur-1224 - him-8

   Plasmid

   #159819

   Purpose

   piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   him-8 piRNAi 21ur-1224
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW1927

   Plasmid

   #163093

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW1886

   Plasmid

   #154333

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

   Plasmid

   #182354

   Purpose

   Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

   Plasmid

   #182356

   Purpose

   Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

   Plasmid

   #159795

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCFJ2214 - Intron GG1 - 900 bp

   Plasmid

   #159880

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 900 bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIC26

   Plasmid

   #44572

   Depositor

   Arshad Desai, Iain Cheeseman

   Article

   Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP, s peptide, and tev

   Expression

   Worm

   Promoter

   pie-1

   Available Since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH3558

   Plasmid

   #179611

   Purpose

   Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   Chrimson
   

   Tags

   Cherry

   Expression

   Worm

   Promoter

   Punc-4

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCZGY3195

    Plasmid

    #172695

    Purpose

    Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Punc-25
    

    Tags

    GFP 1-10

    Expression

    Worm

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD54_01

    Plasmid

    #1507

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, rf2128

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSR35

    Plasmid

    #69256

    Purpose

    phsp16.41::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.41

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGC158

    Plasmid

    #19620

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plag-2::FLP::let-858(3’)
    

    Use

    Flp/frt

    Expression

    Worm

    Promoter

    lag-2 promoter

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBN281

    Plasmid

    #86726

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Plin-31::FLP D5
    

    Expression

    Worm

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS2-his-24::mNeptune

    Plasmid

    #124332

    Purpose

    Expressing Nuclear Localized mNeptune

    Depositor

    Andrew Leifer

    Insert

    mNeptune (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD10_46

    Plasmid

    #1432

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    OriF1<<, OriF1>>, lacZN, unc54N, unc5…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBN258

    Plasmid

    #86721

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Punc-119::FLP D5
    

    Expression

    Worm

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDD316

    Plasmid

    #137020

    Purpose

    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    mTurq2-GLO^SEC^2xHA + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans GLO-optimized mTurq2

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD34_110

    Plasmid

    #1445

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, TmD, lacZN, lacZC

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGC240

    Plasmid

    #19625

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plim-7

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAJ09

    Plasmid

    #14913

    Depositor

    James Lok

    Article

    Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

    Insert

    strongyloides stercoralis G protein alpha subunit promoter
    

    Tags

    GFP(S65C)

    Expression

    Worm

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPD95_81

    Plasmid

    #1497

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    rf8134, IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNM3810

    Plasmid

    #154119

    Purpose

    mec-4 promoter driving QF in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::QF::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS2-his-24::tagBFP

    Plasmid

    #124320

    Purpose

    Expressing Nuclear Localized tagBFP

    Depositor

    Andrew Leifer

    Insert

    tagBFP (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. L3784

    Plasmid

    #1591

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    mec3-1822, mec3Tsa, T7p, gfpN, IVSR, gfpS65C, IVS…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGC320

    Plasmid

    #19639

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R4-R3)

    Use

    Flp/frt, destination vector

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGC93

    Plasmid

    #19628

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt, destination vector

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCM1.120

    Plasmid

    #19600

    Depositor

    Geraldine Seydoux

    Insert

    spn-4 prom in PDONRP4P1R
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

    Plasmid

    #159802

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBCN25-R4R3

    Plasmid

    #26305

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge