8,086 results

1. pDDGFP_LEU2D

   Plasmid

   #58352

   Purpose

   Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

   Depositor

   Simon Newstead

   Article

   Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCYC1m_yeGFP

   Plasmid

   #64389

   Purpose

   encodes yeast enhanced GFP under control of minimal pCYC1 promoter

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pCYC1

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSJ95

   Plasmid

   #104343

   Purpose

   Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   FBP1
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-6xGLY-3xFLAG-hphMX4

   Plasmid

   #20755

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-6xGLY-His-tag-hphMX4

   Plasmid

   #20764

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-6xGLY-His-tag-kanMX6

   Plasmid

   #20763

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBD-pZ-FLAG

   Plasmid

   #183148

   Purpose

   Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Insert

   GAL4 BD
   

   Use

   Yeast two-hybrid

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZalphaB-SapL3

    Plasmid

    #78171

    Purpose

    Expression of Saporin L3 in pichia

    Depositor

    Vern Schramm

    Article

    Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

    Insert

    Saporin L3
    

    Use

    Tags

    alpha factor

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG426-GAL-Alpha-synuclein

    Plasmid

    #181715

    Purpose

    Galactose inducible expression of Alpha-synuclein

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPICZ-pre-Ost1-alphaf(I)-Btl2

    Plasmid

    #117667

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-alphaf(I)-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid

    #181707

    Purpose

    Galactose inducible expression of Kar2-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Kar2-Beta-Amyloid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM-TagRFP657

    Plasmid

    #176563

    Purpose

    Expression of TagRFP657 for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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