8,025 results

1. pRG205

   Plasmid

   #64524

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP112 (pRS406-GFP-ADH1t)

   Plasmid

   #106472

   Purpose

   Vector for creating C-terminal GFP fusion proteins in yeast.

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   None

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS314-4M5.3

   Plasmid

   #45830

   Depositor

   Dane Wittrup

   Insert

   4M5.3
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPM28 (eroGFP CEN/ARS URA3)

   Plasmid

   #20131

   Depositor

   Feroz Papa

   Article

   Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

   Insert

   eroGFP (KAR2 jellyfish, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS306-OsTIR1F74A

   Plasmid

   #198416

   Purpose

   Expresses OsTIR1F74A under the control of ADH1 promoter in budding yeast

   Depositor

   Takumi Kamura

   Article

   Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

   Insert

   OsTIR1F74A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRG713_lexO-HO_LexA-TF_URA3MX

   Plasmid

   #154821

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ube2c

   Plasmid

   #31418

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2C Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pVT100U-mtGFP

   Plasmid

   #45054

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-trp-HYB

    Plasmid

    #64331

    Purpose

    trp1 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of trp1 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRG206

    Plasmid

    #64527

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMZ374

    Plasmid

    #59896

    Purpose

    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2B-55

    Plasmid

    #177924

    Purpose

    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    V9I; F67S; G72P, F86Y; R171K

    Promoter

    TDH3

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. YIplac211-SEC7-mCherry2Bx6

    Plasmid

    #105267

    Purpose

    Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Sec7 portion (SEC7 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSFS2A-CaKan

    Plasmid

    #189565

    Purpose

    Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

    Depositor

    Richard Bennett

    Article

    Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

    Insert

    CaKan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBHM2404

    Plasmid

    #173441

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-NAT1
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS306-URA3-PHIS3-PfV-GS-Sapphire

    Plasmid

    #116932

    Purpose

    Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSC5

    Plasmid

    #188602

    Purpose

    Superior Conjugative Plasmid to Diverse Yeast

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG426GAL-ccdB

    Plasmid

    #14155

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYZ158

    Plasmid

    #98414

    Purpose

    Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. yo-msGFP2

    Plasmid

    #160471

    Purpose

    Encodes a yeast codon optimized version of msGFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    yo-msGFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKanB alpha

    Plasmid

    #223524

    Purpose

    Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCfB3405

    Plasmid

    #106166

    Purpose

    EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBHM2406

    Plasmid

    #173442

    Purpose

    pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    mNeonGreen-CBP-2XFLAG-HYGB
    

    Use

    Tags

    CBP-2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG415GAL-ccdB

    Plasmid

    #14145

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAV106-pTDH3-mCherry-10xSumo-6xSIM

    Plasmid

    #116928

    Purpose

    Yeast integrative vector for expression of the SUMO-SIM complex

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10xSUMO-6xSIM
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge