7,933 results

1. pWL078 MCP-2xGFP

   Plasmid

   #197047

   Purpose

   pCYC-NLS-MCP-2xGFP on pRS305

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   MCP-2xGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

   Plasmid

   #173454

   Purpose

   Contains mScarletI-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mScarlet-I-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pESC-ARG (V5 and HA)

   Plasmid

   #222493

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pESC-MET

   Plasmid

   #222482

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pESC-LYS

   Plasmid

   #222483

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC-TYR

   Plasmid

   #222484

   Purpose

   Co-expression of two genes in yeast with FLAG and MYC tags and TYR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pESC-MET (V5 and HA)

   Plasmid

   #222490

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pESC-LYS (V5 and HA)

   Plasmid

   #222491

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pESC-TYR (V5 and HA)

   Plasmid

   #222492

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   HA and V5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pESC-THR (V5 and HA)

    Plasmid

    #222494

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pESC-THR

    Plasmid

    #222479

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and THR1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pESC-ADE

    Plasmid

    #222480

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and ADE2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pESC-ARG

    Plasmid

    #222481

    Purpose

    Co-expression of two genes in yeast with FLAG and MYC tags and ARG1 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ymTq2-yeVenus pDRF1-GW

    Plasmid

    #168076

    Purpose

    FRET fusion between ymTq2 and yeVenus for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymTq2-yeVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-mScarletI-KanMX6

    Plasmid

    #136690

    Purpose

    Tagging genes in Cterm with mScarletI in fission yeast

    Depositor

    Julien Berro

    Insert

    mScarletI
    

    Use

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Promoter

    None

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLAREG

    Plasmid

    #73044

    Purpose

    Inducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.

    Depositor

    David McMillen

    Article

    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

    Inserts

    EGFP
    LacI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 promoter and hybrid dual-mode promoter-see c…

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIYC08

    Plasmid

    #64743

    Purpose

    Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

    Depositor

    Jens Nielsen

    Article

    Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

    Insert

    ADH2, ALD6, acsL641P, ERG10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p404-MTS-mCherry-GFP1-10

    Plasmid

    #91958

    Purpose

    Expresses mCherry-GFP1-10 in yeast cell mitochondria

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    MTS-mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKK01

    Plasmid

    #64744

    Purpose

    Yeast expression plasmid: phaA, phaB, phaC

    Depositor

    Jens Nielsen

    Article

    Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

    Insert

    phaA, phaB, phaC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFA6a-link-yomCherry-CaURA3

    Plasmid

    #44876

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJRoC30_VP11H

    Plasmid

    #188063

    Purpose

    PROSS-designed versatile peroxidase 11H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Pleurotus ostreatus
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    38 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-his3-P81nmt1-3FLAG

    Plasmid

    #19341

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    his3 (his3 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJH2972

    Plasmid

    #100956

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker

    Depositor

    James Haber

    Article

    Ranjith et al Protocol Exchange (2017)

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HO-pGAL-poly-KanMX4-HO

    Plasmid

    #51664

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHCA/GAL4(1-93).ER.VP16

    Plasmid

    #108216

    Purpose

    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

    Depositor

    Didier Picard

    Article

    Louvion et al Gene 1993 Sep 6;131(1):129-34.

    Insert

    Gal4 DBD-ERalpha HBD-VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH1

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    no

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

    Plasmid

    #86413

    Purpose

    nuclear split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    Nop1pr-GFP11-mCherry-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG426GAL-EGFP

    Plasmid

    #166143

    Purpose

    EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG426GAL-mRuby3

    Plasmid

    #166144

    Purpose

    mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKmK.P12

    Plasmid

    #125045

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    TSA1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

    Plasmid

    #173455

    Purpose

    Contains mScarlet-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDRF1-GW ymScarletI

    Plasmid

    #118452

    Purpose

    Overexpression of yeast codon-optimized mScarletI

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHES839

    Plasmid

    #87941

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PCUP1-DuDre-Atg8-404

    Plasmid

    #69201

    Purpose

    RFP-Atg8 (PAS marker) with improved property

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    DuDre-Atg8
    

    Use

    Tags

    DsRed Express 2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG424GAL-ccdB

    Plasmid

    #14151

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKmK.P6

    Plasmid

    #125039

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    INU1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKmK.P5

    Plasmid

    #125038

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HSP150 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKmK.P18

    Plasmid

    #125051

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    XYL2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge