8,010 results

1. pIII/MS2-1

   Plasmid

   #220629

   Purpose

   Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIII/MS2-2

   Plasmid

   #220630

   Purpose

   For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p426*-SpSgH

   Plasmid

   #136993

   Purpose

   Helper plasmid for SpCas9 gRNA cloning and expression

   Depositor

   Huimin Zhao

   Article

   Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKS1576

   Plasmid

   #239524

   Purpose

   Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

   Depositor

   John Dueber

   Insert

   ScVba2p-mTurquoise2-2xCrk_SH3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJJB1577

   Plasmid

   #218619

   Purpose

   Express Pex5, Pex2, Pex17, and Pex10

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJJB1578

   Plasmid

   #218620

   Purpose

   Express Pex22, Pex6, Pex15, and Pex8

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGADT7-GW

   Plasmid

   #61702

   Purpose

   Gateway compatible Y2H vector - AD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3HR2

    Plasmid

    #220738

    Purpose

    Component of yeast three hybrid system

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAL005

    Plasmid

    #233865

    Purpose

    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRO174

    Plasmid

    #233809

    Purpose

    MoClo-YTK; type3A; ABA systems; Phlf-NES

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    Phlf-NES
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGal4AD-CIB1

    Plasmid

    #28245

    Purpose

    Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    Gal4AD (CIB1, GAL4 Budding Yeast)
    

    Use

    Tags

    CIB1

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGal4BD-CRY2

    Plasmid

    #28243

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EZ-L560

    Plasmid

    #173028

    Purpose

    Encodes OptoAMP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EZ-L175

    Plasmid

    #173025

    Purpose

    Encodes OptoAMP1 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-Sen1

    Plasmid

    #99994

    Purpose

    Shuttle yeast Sen1 into Gateway destination vector

    Depositor

    Susan Baserga

    Article

    Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

    Insert

    Sen1 (SEN1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV10.F3

    Plasmid

    #63199

    Purpose

    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-6xGLY-3xFLAG-HIS3MX6

    Plasmid

    #20753

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEVY-U

    Plasmid

    #51230

    Purpose

    bi-directional expression vector for yeast, constitutively active, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-link-ymScarletI3-Kan

    Plasmid

    #224077

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    ymScarletI3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCTcon2

    Plasmid

    #41843

    Depositor

    Dane Wittrup

    Article

    Chao et al Nat Protoc. 2006;1(2):755-68.

    Insert

    CD20
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA6a-6xGLY-Strep-tagII-kanMX6

    Plasmid

    #20757

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

    Plasmid

    #178991

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYZ960

    Plasmid

    #187972

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIplac211-ABP1-iGFPx6

    Plasmid

    #177099

    Purpose

    Expresses Abp1-iGFPx6 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ABP1 (ABP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDRF1-GW yAT1.03R122KR126K

    Plasmid

    #132782

    Purpose

    Non-binding version of the FRET biosensor for ATP measurements in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

    Insert

    yAT1.03R122KR126K ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R122K, R126K

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge