7,933 results
-
Plasmid#179074PurposeVector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membraneDepositorInsertTEF2p-Scarlet(rfp)-Link-2xPH
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mYFP-kanMX6
Plasmid#105147PurposeYeast gene targetingDepositorInsertmYFP-kanMX6
UseTagsmYFPExpressionBacterial and YeastMutationA206KPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P16
Plasmid#125049PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertFBA1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM1
Plasmid#34607DepositorInsertPKM1 (PKM Human)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-HO
Plasmid#51661Purposeyeast plasmid for integration of target sequence at HO locusDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES836
Plasmid#89195PurposeExpresses mKate2 under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-mKate2
UseTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3R
Plasmid#125065PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1R
Plasmid#125063PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pKT0210
Plasmid#8724DepositorInsertyECFP A206R
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…PromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPT24
Plasmid#184419PurposeTransformation of mitochondrial DNA in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-G418
Plasmid#160007PurposeLow-copy expression vector for K. marxianus, G418 selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarlet1.4-T2A-mTurquoise2
Plasmid#219849Purposeequimolar expression of mScarlet1.4 and mTurquoise2 in budding yeastDepositorInsertmScarlet1.4-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pML107-kanR468
Plasmid#162041PurposeExpresses Cas9 and contains kanR468 guide which targets the kanR geneDepositorInsertkanR468 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6574
Plasmid#106132PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorInsertp1k-GFP-Pep12 (PEP12 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046203
Plasmid#145488PurposeYeast Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS
Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEZY202
Plasmid#18705PurposepEG202 converted Gateway Destination vector for yeast two-hybrid bait vectorDepositorTypeEmpty backboneUseTwo-hybrid bait vectorTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYX113-mtGFP
Plasmid#45049DepositorInsertmtGFP
UseTagsExpressionYeastMutationPromoterGALAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEasyCas9
Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mStayGold-T2A-mCherry
Plasmid#219834Purposeequimolar expression of mStaygold and mCherry in budding yeastDepositorInsertmStayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_EV3
Plasmid#102293PurposeYeast Surface Display of 5Ins_16_EV3DepositorInsertEvolved Insulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWS926
Plasmid#123052PurposeLexA bacterial repressorDepositorInsertLexA-NLS [3a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426 103Q GPD
Plasmid#1184DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p413TEF1-ATR1(pYL573)
Plasmid#178288PurposeExpresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1DepositorInsertATR1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB-TAP
Plasmid#14274DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWS3868
Plasmid#185745PurposeSub-array backbone 4/4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWKS7
Plasmid#139414PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC638
Plasmid#62283PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationNuclease activity has been deactivated by mutatio…PromoterpAdh and pGal10Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p426*-SpSgH
Plasmid#136993PurposeHelper plasmid for SpCas9 gRNA cloning and expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM291-tetR'-tup11Δ70
Plasmid#41028DepositorInserttetR'-tup11Δ70
UseTagsExpressionYeastMutationtup11 starts at aa70PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMJ240: pRS426-His-MBP-MmeFz2_Native
Plasmid#205255PurposeS. cerevisiae expression vector for MmeFz2 native guideDepositorInsertMmeFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVG47
Plasmid#164705PurposeyEVenus under the control of tetOprDepositorInserttetOpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-URA
Plasmid#160009PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Htb2-GFP
Plasmid#117669PurposeColorize the nucleiDepositorInsertHtb2-GFP
UseTagsExpressionYeastMutationContains the gene encoding the Histone Htb2 from …PromoterpGAPAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-Kan
Plasmid#44955PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only