8,265 results
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Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRG226
Plasmid#64529PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-HIS3MX6
Plasmid#20762DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ura4MX6
Plasmid#49184Purposegene deletion in S. pombeDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-SpHis5
Plasmid#219844Purposetagging vector to tag genes with ymScarletI and a HIS5 geneDepositorInsertymScarletI-SpHis5
TagsymScarletIExpressionYeastAvailable SinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-Nb(VHHGFP4)
Plasmid#198412PurposeExpress mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-DuDre-Atg8-404
Plasmid#69201PurposeRFP-Atg8 (PAS marker) with improved propertyDepositorInsertDuDre-Atg8
TagsDsRed Express 2ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
Plasmid#58433PurposeInduces Citrine expression from a promoter containing 2 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)2-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG305GAL-ccdB
Plasmid#14137DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-COF1
Plasmid#31844DepositorAvailable SinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p413_Csy4NLS
Plasmid#103139PurposeExpresses Csy4-NLS under TEF promoterDepositorInsertCsy4
TagsNLSExpressionYeastPromoterTEFAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-VPS21
Plasmid#18842DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM30
Plasmid#133673PurposeIntegration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
ExpressionYeastPromoterpPMA1Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC2_3_dCas9_Mxi1_sgRNA
Plasmid#163708PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressorDepositorInsertsdCas9
Mxi1+SV40 NLS
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-YPT31
Plasmid#18849DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Sen1
Plasmid#99994PurposeShuttle yeast Sen1 into Gateway destination vectorDepositorInsertSen1 (SEN1 Budding Yeast)
ExpressionYeastAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-URA
Plasmid#188932PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416 25Q GAL
Plasmid#1185DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-EGFP-ccdB
Plasmid#14314DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-CaURA3
Plasmid#44873PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNKY51
Plasmid#14839DepositorInserthisG-URA3-hisG
ExpressionYeastAvailable SinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE (V5 and HA)
Plasmid#222489PurposeCo-expression of two genes in yeast with V5 and HA tags and ADE2 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4
Plasmid#185874PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A,H102A
Plasmid#159152PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A,H102A
ExpressionYeastMutationMutated both Met 7 and His 102 of the cyt b562 mo…PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEW8
Plasmid#84238PurposePlasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiaeDepositorInsertHA-mRuby-P2A-eGFP
TagsHA-mRuby and eGFPExpressionYeastPromoterGPDAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCYC2
Plasmid#43970DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterCYC1Available SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-mYFP-hphMX6
Plasmid#105155PurposeYeast gene targetingDepositorInsertmYFP-hphMX6
TagsmYFPExpressionBacterial and YeastMutationA206KAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS646
Plasmid#183175PurposeY2H vector compatible with pJS647 PYR1 insertDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM57
Plasmid#133656PurposeIntegration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.DepositorAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
TagsEGFPExpressionYeastAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastPromoteryeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC7-msCFPx6
Plasmid#105268PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.DepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-DDP1
Plasmid#183950PurposeExpresses yeast DDP1 from yeast GPD promoterDepositorAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03R122K/R126K
Plasmid#28005DepositorInsertAT1.03R122K/R126K
TagsmVenus and mseCFPExpressionYeastMutationR122K, R126KAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only