8,030 results
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Plasmid#186784PurposeExpression of reverse transactivator (CgrtTA)DepositorInsertCgrtTA
UseTagsExpressionYeastMutationPromoterScPGK1pAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pE6n
Plasmid#17460Purposemodified pENTR vector for N-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
p404-MTS-mCherry-GFP1-10
Plasmid#91958PurposeExpresses mCherry-GFP1-10 in yeast cell mitochondriaDepositorInsertMTS-mCherry-GFP1-10
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGAL-PDE1
Plasmid#160388PurposeOverxpression of PDE1 in yeastDepositorInsertPDE1
UseTagsExpressionYeastMutationPromoterGALAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDLC201
Plasmid#113388Purposegalactose-inducible Ty3DepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG201
Plasmid#64394PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-link-yomRuby-Kan
Plasmid#44956PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby with Kan selectionDepositorInsertyomRuby
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-kanMX6
Plasmid#89067Purposegenomic GBP epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBPExpressionYeastMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarletI-KanMX6
Plasmid#136690PurposeTagging genes in Cterm with mScarletI in fission yeastDepositorInsertmScarletI
UseTagsmScarletIExpressionYeastMutationPromoterNoneAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHES830
Plasmid#87944PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTIN7m
Plasmid#162886PurposeOverexpression vector for the fission yeast Schizosaccharomyces pombe (lower strength)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFLAG UBR1 SBX
Plasmid#24506DepositorInsertUBR1 (UBR1 Budding Yeast)
UseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPS3858
Plasmid#113393Purposegalactose-inducible Ty3-his3AI; additional PPTDepositorInsertTy3-his3AI
UseTagshis3AIExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p416 103Q GPD
Plasmid#1180DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)
Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT222
Plasmid#40942DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP467-PACT1(-1-520)-LexA-ER-haVP16
Plasmid#58430PurposeExpresses LexA-ER-VP16 in yeast cellsDepositorInsertLexA-ER-VP16
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCplac22-ENT2
Plasmid#26684DepositorInsertENT2 (ENT2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
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ZJOM140
Plasmid#133668PurposeIntegration of COX4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.DepositorInsertp1k-COX4-mTagBFP2 (COX4 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
UseTagsExpressionYeastMutationPromoterpPMA1Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG10
Plasmid#164702PurposeyEVenus under the control of GALL promoterDepositorInsertGALLpr - yEVenus - CLN2 PEST - ADH1t
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only