7,933 results
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Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natCX
Plasmid#73893Purposefission yeast drug selection markerDepositorInsertnatCX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx1(yeast)
Plasmid#65004PurposeYeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1DepositorInsertGlutaredoxin-1
UseTagsroGFP2ExpressionYeastMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-T
Plasmid#51232Purposebi-directional expression vector for yeast, constitutively active, TRP1 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENO1-iRFP-NATr
Plasmid#129731PurposeIntegration plasmid to overexpress iRFP in Candida albicansDepositorInsertiRFP-Candida albicans codon-optimized
UseTagsExpressionYeastMutationPromoterENO1 from Candida albicansAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HRV-B14_3C
Plasmid#203466PurposeExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHRV-B14 3C protease (HRVBgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
Plasmid#45931DepositorInsertPCP-PS
UseTags2x-yeGFPExpressionYeastMutationPromoterMet25Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIW447-CRISPR XYL2
Plasmid#98906PurposeK. marxianus CRISPR Plasmid for XYL2 editingDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ123: pRS426-His-MBP-SpuFz1_PSP1
Plasmid#205256PurposeS. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)DepositorInsertSpuFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK
Plasmid#183943PurposeExpresses HA-tagged E. coli PPK from yeast GPD promoterDepositorInsertPPK (ppk E. coli)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEZY45
Plasmid#18704PurposepJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vectorDepositorTypeEmpty backboneUseTwo-hybrid prey vectorTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM136
Plasmid#133660PurposeIntegration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-SpHis5
Plasmid#219844Purposetagging vector to tag genes with ymScarletI and a HIS5 geneDepositorInsertymScarletI-SpHis5
UseTagsymScarletIExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pHLM
Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-TRP1
Plasmid#11007DepositorInsertTRP1 (TRP1 Budding Yeast)
UseTagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 without stop codonPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
UseTagsExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372APromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastMutationPromoteryeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterATG8Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046647
Plasmid#153940PurposeYeast expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM2
Plasmid#198413PurposeExpress mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM2)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-HphMx
Plasmid#122922PurposeTRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceJuly 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03R122K/R126K
Plasmid#28005DepositorInsertAT1.03R122K/R126K
UseTagsmVenus and mseCFPExpressionYeastMutationR122K, R126KPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMAL728
Plasmid#169152PurposeEncodes OptoQ-AMP6DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationA79QPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P15
Plasmid#125048PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertTDH3 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-EGFP
Plasmid#14196DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-KanMx
Plasmid#122923PurposeTRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM1959
Plasmid#41837DepositorInsertSEC63-mRFP
UseTagsmRFPExpressionYeastMutationPromoterAvailable sinceJan. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-Cerulean-ccdB
Plasmid#14420DepositorTypeEmpty backboneUseGateway destinationTagsCeruleanExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
EZ-L545
Plasmid#173024PurposeEncodes OptoEXP2 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-NatMx
Plasmid#122924PurposeTRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LHP306 - Ub - no Lys
Plasmid#32150DepositorUseTagsExpressionYeastMutationNo Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only