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7,933 results

Showing: 1201 - 1240 of 7933 results
  1. pTPGI_dCas9

    Plasmid
    #64279
    Purpose
    encodes yeast-optimized dCas9 synthetic transcription factor
    Depositor
    Insert
    Yeast-optimized dCas9
    Use
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    D10A & H840A relative to S. pyogenes Cas9
    Promoter
    pTPGI
    Available since
    May 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pDB4280

    Plasmid
    #98699
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pAG416GAL10-HRV-B14_3C

    Plasmid
    #203466
    Purpose
    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker
    Depositor
    Insert
    HRV-B14 3C protease (HRVBgp1 )
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pHES795

    Plasmid
    #87943
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Insert
    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pEZY45

    Plasmid
    #18704
    Purpose
    pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector
    Depositor
    Type
    Empty backbone
    Use
    Two-hybrid prey vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
  7. ZJOM136

    Plasmid
    #133660
    Purpose
    Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Insert
    p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pFA6a-link-ymScarletI-SpHis5

    Plasmid
    #219844
    Purpose
    tagging vector to tag genes with ymScarletI and a HIS5 gene
    Depositor
    Insert
    ymScarletI-SpHis5
    Use
    Tags
    ymScarletI
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pYES2.1-TRP1

    Plasmid
    #11007
    Depositor
    Insert
    TRP1 (TRP1 Budding Yeast)
    Use
    Tags
    V5 and his6
    Expression
    Bacterial and Yeast
    Mutation
    ORF-TRP1 without stop codon
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
  10. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pGBW-m4046647

    Plasmid
    #153940
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3C-like proteinase
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pYeDP60-hATP13A2-E343A

    Plasmid
    #171378
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    E343A
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. FRP2374

    Plasmid
    #127579
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker
    Depositor
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1201 - 1240 of 7933 results