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Yeast Expression

8,010 results

Showing: 1201 - 1240 of 8010 results

  2. pILGFP95

    Plasmid
    #83550
    Purpose
    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Insert
    PDA1 promoter-yEGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PDA1
    Available since
    Dec. 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. p404GAL1

    Plasmid
    #17417
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17417-plasmid-map-sequence-id-8592

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  10. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  13. ZJOM10

    Plasmid
    #133639
    Purpose
    Integration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-Vps4-GFP (VPS4 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133639-plasmid-map-sequence-id-265901

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  15. RIB VP64 R1

    Plasmid
    #161493
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2
    Depositor
    Diethard Mattanovich, Brigitte Gasser
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161493-plasmid-map-sequence-id-326164

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  20. pTPGI_dCas9

    Plasmid
    #64279
    Purpose
    encodes yeast-optimized dCas9 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    Yeast-optimized dCas9
    Use
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    D10A & H840A relative to S. pyogenes Cas9
    Promoter
    pTPGI
    Available since
    May 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    64279-plasmid-map-sequence-id-107786

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  24. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

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  25. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  26. pE5c

    Plasmid
    #17465
    Purpose
    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17465-plasmid-map-sequence-id-8633

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  31. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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  39. pDB4282

    Plasmid
    #98701
    Purpose
    A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98701-plasmid-map-sequence-id-182827

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Showing: 1201 - 1240 of 8010 results