8,265 results
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Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p426 25Q GPD
Plasmid#1181DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLK
Plasmid#64186Purposeharbours marker genes LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
ExpressionYeastPromoterPGK1Available SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-his3-P81nmt1-3FLAG
Plasmid#19341DepositorInserthis3 (his3 Fission Yeast)
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3
Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsGFPMUT3ExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-KanMX6
Plasmid#69851PurposeC-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Mup1-mScarlet2I
Plasmid#140152PurposePlasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2IDepositorInsertMUP1
ExpressionYeastAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNXRVc-HACre
Plasmid#78400Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
natMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK010-3a-Glucoamylase-αMFΔ
Plasmid#84969PurposeType 3a, Glucoamylase followed by αMFΔ - secretion signalDepositorInsertGlucoamylase followed by αMFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP416
Plasmid#26842DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HyTkAX
Plasmid#73898Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE URA3
Plasmid#122882PurposeURA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB-DsRed
Plasmid#14370DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179068PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid dropletDepositorInsertRPL18Bp-PDR16-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)
Plasmid#179063PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosomeDepositorInsertRPL18Bp-Scarlet(rfp)-Link-YPT7
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT
Plasmid#140594PurposeExpression in S. cerevisiaeDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAPZ-STE2-1D4
Plasmid#133423PurposeYeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
ExpressionYeastMutationC59S, C252A. No alpha factor secretion signal in …Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
M3927 ura3::KanMX3 Disruptor Converter
Plasmid#51682PurposeYeast marker swap plasmid for converting URA3 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
S3_001_026_MV2
Plasmid#134422PurposepYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
TagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKT0210
Plasmid#8724DepositorInsertyECFP A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…Available SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xHAExpressionYeastPromoterGPDAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-DsRed
Plasmid#14364DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1
Plasmid#203163PurposeYeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase-SAT1
Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13(315)
Plasmid#122072PurposeExpressing GFP-Atg13DepositorInsertATG13
TagsGFPExpressionYeastAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pVG47
Plasmid#164705PurposeyEVenus under the control of tetOprDepositorInserttetOpr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
ExpressionYeastPromoterTetO7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWKS10
Plasmid#139417PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.DepositorInsertClover
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS406-CMV-lacI-3FLAG
Plasmid#83410PurposeExpression of lacI in yeastDepositorInsertlacI repressor
Tags3xFLAG and NLSExpressionYeastPromoterCMVAvailable SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Gal TDP-43 YFP
Plasmid#27468DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P3nmt1-3FLAG
Plasmid#19345DepositorInsertnatMX6
ExpressionYeastAvailable SinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
ExpressionYeastAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK
Plasmid#183943PurposeExpresses HA-tagged E. coli PPK from yeast GPD promoterDepositorAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046663
Plasmid#153857PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits