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Addgene

7,933 results

Showing: 1751 - 1800 of 7933 results
  1. pCfB6638

    Plasmid
    #106165
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance
    Depositor
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pRS423-mitoT

    Plasmid
    #174525
    Purpose
    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes
    Depositor
    Insert
    Yeast mitochondrial intermembrane tether
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Yeast
    Mutation
    Promoter
    MET25
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pDRFLIP43

    Plasmid
    #65519
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-edCer-cMyc
    Expression
    Yeast
    Mutation
    Promoter
    pPMA1
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pDRFLIP39

    Plasmid
    #65517
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Yeast
    Mutation
    Promoter
    pPMA1
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pYSD153

    Plasmid
    #212929
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Insert
    mRuby2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pYSD141

    Plasmid
    #212928
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Insert
    mRuby2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. FRP1999

    Plasmid
    #159308
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. MmSphk2

    Plasmid
    #118598
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Insert
    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pAG416-GAL-TDP-43

    Plasmid
    #181709
    Purpose
    Galactose inducible expression of TDP-43
    Depositor
    Insert
    TDP-43 (TARDBP Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pILAC3

    Plasmid
    #185890
    Purpose
    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus
    Depositor
    Insert
    see comments
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERG20 F96C; Xd.CRtYB E83K
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pIT6EG7ml

    Plasmid
    #185886
    Purpose
    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).
    Depositor
    Insert
    see comments
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERG20 F96W N127W
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Mutation
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pGBW-m4046483

    Plasmid
    #153868
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp3
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. pFA6a-link-ymVenus-URA3

    Plasmid
    #168058
    Purpose
    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP
    Depositor
    Insert
    ymVenus
    Use
    Tags
    ymVenus
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  16. pCfB6627

    Plasmid
    #106159
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance
    Depositor
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1751 - 1800 of 7933 results