8,010 results

1. pAG426GAL-ccdB-DsRed

   Plasmid

   #14371

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUG-amdSYM-mak10TR

   Plasmid

   #75433

   Purpose

   Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

   Depositor

   John McCusker

   Inserts

   MAK10TR
   ApR
   amdSYM
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminal 740 bp of MAK10 ORF

   Promoter

   Native promoter and PTEF

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB2791

   Plasmid

   #63654

   Purpose

   pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKT0209

   Plasmid

   #8730

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEGFP
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KBB364

   Plasmid

   #185083

   Purpose

   RFA1 fused to GFP under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JME4390

   Plasmid

   #129656

   Purpose

   LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p404-Grx5-GFP1-10

   Plasmid

   #91952

   Purpose

   Expresses GFP1-10 in the mitochondrial matrix

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   Grx5-GFP1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pABY-c46

    Plasmid

    #201774

    Purpose

    Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LPH2781 - Ub-K63R

    Plasmid

    #32160

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. YIplac211-iGFP-TLG1

    Plasmid

    #105261

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIPlac204TKC-E2-Crimson-HDEL

    Plasmid

    #38771

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Use

    Tags

    Linker peptide and yeast ER retention signal sequ…

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-yomKeima-Kan

    Plasmid

    #44902

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP-ATG13(315)

    Plasmid

    #122072

    Purpose

    Expressing GFP-Atg13

    Depositor

    Daniel Klionsky

    Article

    Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

    Insert

    ATG13
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

    Plasmid

    #173456

    Purpose

    Contains mScarletH-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-H-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICE URA3

    Plasmid

    #122882

    Purpose

    URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG425GPD-ccdB-DsRed

    Plasmid

    #14370

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYX122-mtGFP

    Plasmid

    #45048

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pLexA::Ras

    Plasmid

    #11340

    Depositor

    Michael Wigler

    Article

    Chang et al Cell. 1994 Oct 7. 79(1):131-41.

    Insert

    Ras1 (ras1 Fission Yeast)
    

    Use

    Tags

    LexA DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pDRF1 Cyterm-ytd8oxStayGold

    Plasmid

    #219853

    Purpose

    expression of Cyterm-ytd8oxStayGold in budding yeast

    Depositor

    Bas Teusink

    Insert

    Cyterm-ytd8oxStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pAG304GAL-ccdB

    Plasmid

    #14135

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS402-nicastrin/APH-1a-HA

    Plasmid

    #207804

    Purpose

    Yeast integrative vector for expression of Nicastrin and APH-1a-HA

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits nicastrin and Aph1a
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEV-G2-Ph

    Plasmid

    #46816

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBW-m4046708

    Plasmid

    #149173

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. M3925 trp1::KanMX3 Disruptor Converter

    Plasmid

    #51681

    Purpose

    Yeast marker swap plasmid for converting Trp1 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEVY-4420-mCherry

    Plasmid

    #218828

    Purpose

    Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    4-4-20 scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEVY-pT231 scFv-GFP

    Plasmid

    #218827

    Purpose

    Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    pT231 tau scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pK3FS

    Plasmid

    #85776

    Purpose

    N-ICE plasmid pK3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGI3EM09

    Plasmid

    #135482

    Purpose

    Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunHsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pACT2.2gtwy

    Plasmid

    #11346

    Depositor

    Guy Caldwell

    Insert

    gateway cassette
    

    Use

    Tags

    Gal4 Activation Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pAG425GPD-EGFP-ccdB

    Plasmid

    #14322

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETconNK-TEM1(S70A,D179G)

    Plasmid

    #81166

    Purpose

    pETconNK with TEM1(S70A,D179G) beta-lactamase

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    c-myc

    Expression

    Bacterial and Yeast

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    GAL1 and gal1

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPS2351

    Plasmid

    #1352

    Depositor

    Pamela Silver

    Article

    Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

    Insert

    MGA2 (MGA2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

39. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFA6a-link-yoLSS-mKate2-Kan

    Plasmid

    #44943

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge