8,016 results

1. pRS426-GFP-PLCdelta-PH

   Plasmid

   #71375

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   PLCdelta-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain

   Promoter

   Available since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pAG425GAL-ccdB-HA

   Plasmid

   #14249

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CUP Cyc8 YFP p316

   Plasmid

   #15594

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   CYC8 (CYC8 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pILGFPH7

   Plasmid

   #83554

   Purpose

   Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   ADH2 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH2

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2337

   Plasmid

   #67555

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDRF1-GW (n1)StayGold-T2A-mCherry

   Plasmid

   #205495

   Purpose

   Expression of (n1)StayGold-T2A-mCherry in yeast

   Depositor

   Bas Teusink

   Insert

   (n1)StayGold-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT25_GalL_BE3

   Plasmid

   #143736

   Purpose

   Expresses BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV0356

   Plasmid

   #133468

   Purpose

   Integration into fission yeast genome at ade6 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pESC-URA-Rnq1-GFP

    Plasmid

    #21051

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pSM1960

    Plasmid

    #41842

    Depositor

    Susan Michaelis

    Article

    Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

    Insert

    SEC63-mRFP
    

    Use

    Tags

    mRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTCW007

    Plasmid

    #83525

    Purpose

    pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

    Insert

    pFUS1J2-mfalpha1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-yoGFPgamma-SpHis5

    Plasmid

    #44859

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoGFPgamma
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIplac204-TC-OM45-FKBPx4

    Plasmid

    #104472

    Purpose

    Integrating plasmid for overexpression of OM45-FKBPx4.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    OM45p (OM45 Budding Yeast)
    

    Use

    Tags

    FKBPx4- HA

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LHP2260 - HA-INP53

    Plasmid

    #32198

    Depositor

    Linda Hicke

    Insert

    INP53 (INP53 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Lac

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

17. p406MET3

    Plasmid

    #17436

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET3 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRoC30_VP5H

    Plasmid

    #188061

    Purpose

    PROSS-designed versatile peroxidase 5H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Pleurotus ostreatus
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    43 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWKS5

    Plasmid

    #139412

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Citrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYES-mtBFP

    Plasmid

    #45055

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pAG413GAL-ccdB-EGFP

    Plasmid

    #14189

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG303GPD-ccdB-EGFP

    Plasmid

    #14182

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RS314-H

    Plasmid

    #17541

    Depositor

    Bert Vogelstein

    Article

    Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

    Insert

    Gal1-p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R273H

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pDRFLIP34

    Plasmid

    #65512

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPM56

    Plasmid

    #22855

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    eroGFP & UPR-RFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG424GPD-EGFP-ccdB

    Plasmid

    #14320

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Dcp2 FL

    Plasmid

    #163574

    Purpose

    express wild type Dcp2 at endogenous level in S.cerevisiae

    Depositor

    Roy Parker

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    DCP2 promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YIp ADE2-URA3

    Plasmid

    #122907

    Purpose

    ADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCEV-G3-Ph

    Plasmid

    #46818

    Purpose

    TEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and GAL10

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ClhN-p1k-Vrg4-V5-APEX2-URA

    Plasmid

    #207071

    Purpose

    Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Vrg4
    

    Use

    Tags

    V5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pVRG4

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ClhN-4V5-APEX2-URA

    Plasmid

    #207056

    Purpose

    For ORF tagging with 4V5-APEX2

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    4xV5-APEX2
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ClhN-pADH3-4V5-APEX2-URA

    Plasmid

    #207063

    Purpose

    Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    4xV5-APEX2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ClhN-pADH3-Tgl3-4V5-APEX2-URA

    Plasmid

    #207066

    Purpose

    Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Tgl3
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSUC2::GNSI (NrsR)

    Plasmid

    #121537

    Purpose

    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPTK006-3a-αMF no EAEA

    Plasmid

    #84965

    Purpose

    Type 3a, α-mating factor no EAEA - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor no EAEA secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-link-yomEos2-SpHis5

    Plasmid

    #44849

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Gma-pCAMBIA-1301-STTM171

    Plasmid

    #84080

    Purpose

    Disturb the expression of miR171 in soybean

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR171
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge