7,933 results
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Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
M3926 leu2::KanMX3 Disruptor Converter
Plasmid#51680PurposeYeast marker swap plasmid for converting LEU2 to KanMX3DepositorInsertKanMX3
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-SirT1
Plasmid#51746Purposeoverexpression of human SirT1 in S. cerevisiae, HIS3 markerDepositorInsertSirT1 (SIRT1 Human)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGAN200
Plasmid#129203PurposeyTRAP sensor of [PSI+], detects aggregation of Sup35DepositorInsertSup35NM (SUP35 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationN terminal and middle domainsPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046409
Plasmid#153939PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC-X4
Plasmid#176154Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII1
Plasmid#176159Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS316CUP RNQ CFP
Plasmid#1171DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
416Gal-SV40NLS-FUS-YFP
Plasmid#29595DepositorInsertNLS-FUS (FUS Human)
UseTagsSV40 NLS and YFPExpressionYeastMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI3
Plasmid#176157Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-TagRFP-SpHis5
Plasmid#44854PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selectionDepositorInsertyoPA-TagRFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPTK003-2-pENO1
Plasmid#84962PurposeType 2, pENO1, Enolase 1 promoterDepositorInsertEnolase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (2350 g to c) and BsmBI site re…PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-p53
Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (TP53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
UseTagsFLAGExpressionYeastMutationPromoterGAL10Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRS426 TEF mCherry
Plasmid#129433Purposeexpress mcherryDepositorInsertmcherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0710
Plasmid#133517PurposeExpression of mCherry tagged beta-tubulin to monitor microtubulesDepositorInsertpAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0749
Plasmid#133483PurposeExpression in fission yeast from promoter ptdh1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE HIS3
Plasmid#122877PurposeHIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pIM690
Plasmid#74638Purposealias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymVenus-CaURA3
Plasmid#118459PurposeYeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selectionDepositorInsertymVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAG416GPD-Cerulean-SEC4
Plasmid#18844DepositorInsertSEC4 (SEC4 Budding Yeast)
UseTagsCeruleanExpressionYeastMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0663
Plasmid#133478PurposeExpression in fission yeast from promoter pact1, integrated at his5 locusDepositorInsertpHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL2/GZ
Plasmid#1282DepositorInsertGRE-lacZ
UseTagsglucocorticoid response elemen-lacZExpressionYeastMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-EGFP-ccdB
Plasmid#14312DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDH22
Plasmid#83798PurposeUseful for tagging yeast proteins with N-terminal YFP tag; G418 selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAH5
Plasmid#41829PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7,TEFAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB6578
Plasmid#106130PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE1c
Plasmid#17461Purposemodified pENTR vector for C-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only