7,935 results

1. pU-mRuby2

   Plasmid

   #176549

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH-mRuby2

   Plasmid

   #176547

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCTCON2-GYB

   Plasmid

   #160927

   Purpose

   Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Inserts

   BFP
   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pV1093:SpRY

   Plasmid

   #172188

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG413GAL-ccdB-TAP

   Plasmid

   #14261

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-link-yomKO2-Kan

   Plasmid

   #44958

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDDGFP-Leu2d-GgKDELR2

   Plasmid

   #123618

   Purpose

   Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV0714

   Plasmid

   #133479

   Purpose

   Expression in fission yeast from promoter pact1, integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUDE710

   Plasmid

   #103020

   Purpose

   expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKT0175

    Plasmid

    #8733

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECitrine
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S65G, V68L, Q69M, S72A, T203Y

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSNR52-sgTET

    Plasmid

    #46923

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgRNA targeting endogenous TRE elements of pTET07 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YEpURA-CUP1-HBTubiquitin

    Plasmid

    #26900

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

    Insert

    HBT-tagged ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pK3F-GAL1

    Plasmid

    #85772

    Purpose

    N-ICE plasmid pK3F-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-yomRuby-SpHis5

    Plasmid

    #44843

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKmK.I2R

    Plasmid

    #125064

    Purpose

    Storage plasmid for K.marxianus right homology arm

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Right homology arm for targeting integration to chromosome V: 23743..21744
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Fox, Thomas Mason

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pKmK.I2L

    Plasmid

    #125031

    Purpose

    Storage plasmid for K.marxianus left homology arm; type 1 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Left homology arm for targeting integration to chromosome V: 23743..21744
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG424GPD-ccdB-EGFP

    Plasmid

    #14200

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIplac204-pOst1-ESCargo

    Plasmid

    #115420

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG306GPD-ccdB-EGFP

    Plasmid

    #14188

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG413GAL-ccdB-HA

    Plasmid

    #14237

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG414GAL-ccdB-HA

    Plasmid

    #14239

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCETF

    Plasmid

    #19783

    Depositor

    Jumi Shin

    Article

    Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

    Type

    Empty backbone

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GAL FLAG25Q+ProGFPp303

    Plasmid

    #15572

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q + Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCT-4M5.3

    Plasmid

    #41844

    Depositor

    Dane Wittrup

    Article

    Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

    Insert

    4M5.3
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-yEPAGFP-CaUra3

    Plasmid

    #44649

    Depositor

    Kurt Thorn

    Insert

    yEPAGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p404MET25

    Plasmid

    #17420

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mEGFP-FKBP(M)x4

    Plasmid

    #85004

    Purpose

    Expresses mEGFP-FKBP(M)x4

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    EGFP-FKBP(M)x4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFTK043

    Plasmid

    #171315

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eGFP fluorescent reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFA6a-kanMX6-P81nmt1-mYFP

    Plasmid

    #105144

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P81nmt1-mYFP
    

    Use

    Tags

    81nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    81nmt1

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFA6a-6xGLY-S-tag-kanMX6

    Plasmid

    #20766

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 303Gal-FUS

    Plasmid

    #29614

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Use

    Tags

    His6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PhyB-mCherry-PEX3

    Plasmid

    #51572

    Purpose

    Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    PEX3 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YN2_1_IL50_HOlocus

    Plasmid

    #194426

    Purpose

    Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKL08

    Plasmid

    #104432

    Purpose

    pHuji in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGBW-m4046633

    Plasmid

    #149161

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge